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Pregled bibliografske jedinice broj: 615283

Effect of cysteamine supplementation during in vitro culture of early stage bovine embryos on blastocyst rate and quality


Lojkić, Martina; Getz, Iva; Samardžija, Marko; Matković, Mario; Bačić, Goran; Karadjole, Tugomir; Maćešić, Nino; Folnožić, Ivan; Špoljarić, Branimira
Effect of cysteamine supplementation during in vitro culture of early stage bovine embryos on blastocyst rate and quality // Acta veterinaria (Brno), 81 (2012), 3; 229-234 doi:10.2754/avb201281030229 (međunarodna recenzija, članak, znanstveni)


Naslov
Effect of cysteamine supplementation during in vitro culture of early stage bovine embryos on blastocyst rate and quality

Autori
Lojkić, Martina ; Getz, Iva ; Samardžija, Marko ; Matković, Mario ; Bačić, Goran ; Karadjole, Tugomir ; Maćešić, Nino ; Folnožić, Ivan ; Špoljarić, Branimira

Izvornik
Acta veterinaria (Brno) (0001-7213) 81 (2012), 3; 229-234

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Oxidative stress; antioxidants; embryo yield; differential staining; total cell number

Sažetak
The aim of this study was to evaluate whether the addition of cysteamine to the in vitro culture media enhances the yield, hatching rate, total cell number and inner cell mass/total cell number ratio of bovine embryos. A total of 933 bovine oocytes collected from ovaries of 60 slaughtered donors were subjected to in vitro maturation and in vitro fertilization. Following fertilization, embryos were cultured in synthetic oviductal fluid without glucose. After 24 h embryos were transferred into synthetic oviductal fluid with 1.5 mM glucose and 0 (control), 50, 100 and 200 µM of cysteamine. After 48 h, the embryos were transferred into synthetic oviductal fluid with glucose but without cysteamine and cultured until Day 9. The number of cleaved embryos on Day 2, the total number of blastocysts on Day 7 and the number of hatched blastocysts on Day 9 were calculated. Differential staining of inner cell mass and trophectoderm cells of blastocysts were performed on Day 7 and Day 9 of in vitro culture. Supplementation of in vitro culture media with 100 µM cysteamine increased the blastocyst yield (P < 0.05) without affecting the hatching rate. Furthermore, the embryos cultured in the presence of 100 µM cysteamine had significantly higher number of inner cell mass cells (P < 0.05) and the proportion of inner cell mass cells (P < 0.05) compared with the controls. The results of the present study demonstrated that the addition of 100 µM cysteamine to the in vitro culture media improved blastocyst production rate and enhance embryo quality, which could lead to the improvement of the in vitro culture system for bovine embryos.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina



POVEZANOST RADA


Projekt / tema
053-0481186-1185 - Primjena molekularnih analiza u proizvodnji goveđih zametaka in vitro (Iva Getz, )
053-0532052-2040 - Značaj kontrole puerperija u poboljšanju reproduktivne učinkovitosti krava (Tomislav Dobranić, )
053-0532052-2044 - Program suzbijanja mastitisa (Goran Bačić, )
053-0532052-2046 - Smanjena plodnost u mliječnih goveda (Darko Gereš, )

Ustanove
Veterinarski fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Uključenost u ostale bibliografske baze podataka:


  • AGRICOLA
  • BIOSIS Previews (Biological Abstracts)
  • CAB Abstracts
  • FSTA: Food Science and Technology Abstracts
  • MEDLINE
  • Zoological Record


Citati