Prevention of genotoxicity of mycotoxins ochratoxin A and fumonisin B1 by chlorophyllin (CROSBI ID 593895)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Domijan, Ana-Marija ; Gajski, Goran ; Novak, Ivana ; Gerić, Marko ; Garaj-Vrhovac, Vera
engleski
Prevention of genotoxicity of mycotoxins ochratoxin A and fumonisin B1 by chlorophyllin
Chlorophyllin (CHL) is a semi-synthetic derivative of the natural green pigment chlorophyll shown to have beneficial properties on human health. Present study aimed to investigate its possible protective effects toward mycotoxins ochratoxin A (OTA) and fumonisin B1 (FB1), frequent contaminants of crop round the world. Both mycotoxins are implicated in tumor development in humans ; OTA in development of urinary tract tumors and FB1 in oesophageal cancer and primary liver cancer. In this study toxicological profile of CHL (0.1 to 100 µg/ml) was evaluated by measuring the cytotoxic and genotoxic (comet and micronucleus assay) potential of CHL on primary human peripheral blood lymphocytes (HPBLs). CHL, in concentration range tested, had no impact on cell viability and genome damage. In addition CHL did not induce lipid peroxidation in HPBLs and exhibited free radical scavenging activity in DPPH assay. As expected, both mycotoxins OTA (4 µM) and FB1 (20 µg/ml) produced DNA damage in HPBLs already after 1 h exposure. Possible protective role of CHL against OTA and FB1 induced cytogenotoxicity was followed by co-exposing HPBLs to CHL and OTA or FB1. Results demonstrated that CHL was able to protect against cell and DNA damage induced by OTA and FB1. In conclusion, we have shown that CHL alone is safe from the aspect of cytogenotoxicity in the concentration range tested, and could prevent cytogenotoxic potential of OTA and FB1 probably acting as an antioxidants. Thus, CHL could be a perfect candidate for exploring its possible use in anti-cancer therapy.
mycotoxins; chlorophyllin; human lymphocytes
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Podaci o prilogu
48-x.
2012.
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objavljeno
Podaci o matičnoj publikaciji
Periodicum biologorum
Levanat, Sonja ; Levačić Cvok, Mirela ; Musani, Vesna ; Car, Diana ; Osmak, Maja ; Herak Bosnar, Maja ; Slade Neda ; Stojanović, Nikolina
Zagreb: Hrvatsko prirodoslovno društvo ; Institut Ruđer Bošković
0031-5362
Podaci o skupu
Second Meeting of the Croatian Association for Cancer Research with International Participation
poster
08.11.2012-09.11.2012
Zagreb, Hrvatska
Povezanost rada
Kemija, Javno zdravstvo i zdravstvena zaštita, Biologija