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Pregled bibliografske jedinice broj: 609822

CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages


Poglitsch, M.; Weichhart, T.; Hecking, M.; Werzowa, J.; Katholnig, K.; Antlanger, M.; Krmpotić, Astrid; Jonjić, Stipan; Hörl, W.; Zlabinger, G. et al.
CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages // American journal of transplantation, 12 (2012), 6; 1458-1468 doi:10.1111/j.1600-6143.2012.04002.x (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 609822 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages

Autori
Poglitsch, M. ; Weichhart, T. ; Hecking, M. ; Werzowa, J. ; Katholnig, K. ; Antlanger, M. ; Krmpotić, Astrid ; Jonjić, Stipan ; Hörl, W. ; Zlabinger, G. ; Puchhammer, E. ; Säemann, M.

Izvornik
American journal of transplantation (1600-6135) 12 (2012), 6; 1458-1468

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cytomegalovirus; interferon-beta; macrophages; rapamycin; renal transplantation

Sažetak
Human cytomegalovirus (CMV) remains one of the most important pathogens following solid-organ transplantation. Mounting evidence indicates that mammalian target of rapamycin (mTOR) inhibitors may decrease the incidence of CMV infection in solidorgan recipients. Here we aimed at elucidating the molecular mechanisms of this effect by employing a human CMV (HCMV) infection model in human macrophages, since myeloid cells are the principal in vivo targets of HCMV. We demonstrate a highly divergent host cell permissiveness for HCMV with optimal infection susceptibility in M2 but not M1 polarized macrophages. Employing an ultrahigh purified HCMV stock we observed rapamycin-independent viral entry and induction of IFN-b transcripts, but no proinflammatory cytokines or mitogen-activated protein kinases and mTOR activation early after infection. However, in the late infection phase, sustained mTOR activation was observed in HCMV- infected cells and was required for efficient viral protein synthesis including the viral late phase proteins pUL-44 and pp65. Accordingly, rapamycin strongly suppressed CMV replication 3 and 5 days postinfection in macrophages. In conclusion, these data indicate that mTOR is essential for virus replication during late phases of the viral cycle in myeloid cells and might explain the potent anti-CMV effects of mTOR inhibitors after organ transplantation.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
062-0621261-1263 - Molekularni mehanizmi citomegalovirusnog izmicanja imunološkom nadzoru (Jonjić, Stipan, MZOS ) ( POIROT)
062-0621261-1268 - Uloga imunosubverzivnih citomegalovirusnih gena u latenciji (Krmpotić, Astrid, MZOS ) ( POIROT)

Ustanove:
Medicinski fakultet, Rijeka

Profili:

Avatar Url Stipan Jonjić (autor)

Avatar Url Astrid Krmpotić (autor)

Citiraj ovu publikaciju

Poglitsch, M.; Weichhart, T.; Hecking, M.; Werzowa, J.; Katholnig, K.; Antlanger, M.; Krmpotić, Astrid; Jonjić, Stipan; Hörl, W.; Zlabinger, G. et al.
CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages // American journal of transplantation, 12 (2012), 6; 1458-1468 doi:10.1111/j.1600-6143.2012.04002.x (međunarodna recenzija, članak, znanstveni)
Poglitsch, M., Weichhart, T., Hecking, M., Werzowa, J., Katholnig, K., Antlanger, M., Krmpotić, A., Jonjić, S., Hörl, W. & Zlabinger, G. (2012) CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages. American journal of transplantation, 12 (6), 1458-1468 doi:10.1111/j.1600-6143.2012.04002.x.
@article{article, year = {2012}, pages = {1458-1468}, DOI = {10.1111/j.1600-6143.2012.04002.x}, keywords = {Cytomegalovirus, interferon-beta, macrophages, rapamycin, renal transplantation}, journal = {American journal of transplantation}, doi = {10.1111/j.1600-6143.2012.04002.x}, volume = {12}, number = {6}, issn = {1600-6135}, title = {CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages}, keyword = {Cytomegalovirus, interferon-beta, macrophages, rapamycin, renal transplantation} }
@article{article, year = {2012}, pages = {1458-1468}, DOI = {10.1111/j.1600-6143.2012.04002.x}, keywords = {Cytomegalovirus, interferon-beta, macrophages, rapamycin, renal transplantation}, journal = {American journal of transplantation}, doi = {10.1111/j.1600-6143.2012.04002.x}, volume = {12}, number = {6}, issn = {1600-6135}, title = {CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages}, keyword = {Cytomegalovirus, interferon-beta, macrophages, rapamycin, renal transplantation} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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