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Zebrafish genome stability after exposure to model genotoxicants (CROSBI ID 592748)

Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija

Šrut Maja ; Štambuk Anamaria ; Bourdineaud Jean-Paul ; Klobučar Göran Zebrafish genome stability after exposure to model genotoxicants // Comparative biochemistry and physiology. Part A, Molecular & integrative physiology / Mommsen, T.P. ; Walsh, P.J. (ur.). 2012. str. 15-15

Podaci o odgovornosti

Šrut Maja ; Štambuk Anamaria ; Bourdineaud Jean-Paul ; Klobučar Göran

engleski

Zebrafish genome stability after exposure to model genotoxicants

Sublethal exposure to a variety of genotoxic agents in the environment may directly or indirectly influence genome integrity of exposed organisms and for that reason various methods for detection of DNA damage were developed. In this study we have tested and compared three different methods for detection of DNA damage and alterations using in vivo and in vitro zebrafish models. The exposure of embryonic cell line (PAC-2) and zebrafish embryos to genotoxic agents (benzo[a]pyrene (B[a]P) and ethyl methanesulfonate (EMS)) was carried out for 6 days followed by 6 days of recovery period. Genome stability of tested models was assessed using Comet assay, quantitative RAPD (random amplified polymorphic DNA) and AFLP (amplified fragment length polymorphism). Comet assay revealed significant increase of DNA damage in zebrafish embryos following both toxicant treatments and this damage remained unrepaired after the recovery period. However, in PAC-2 cell line significant increase of DNA damage was observed only upon EMS treatment. RAPD showed significant increase in number of hybridization sites in DNA of zebrafish embryos and decrease in DNA of PAC-2 cell line upon exposure to lower B[a]P concentration, while AFLP revealed changes in profiles, such as loss or appearance of fragments as well as changes in fragment amplification intensity, following both toxicants treatment. Our results indicate that RAPD and AFLP are sensitive methods for detection of DNA alterations since they showed changes in the genome even when Comet assay indicated lack of significant damage. This could be due to the presence of more persistent DNA changes such as formation of DNA adducts, mutations or conformational and structural changes in the DNA, all undetected in the Comet assay. These results thus encourage the simultaneous use of various methods and models for revealing the consequences of genotoxic insult and suggest that quantitative RAPD and AFLP could be useful biomarkers in such surveys.

Danio rerio; genotoxicity; Comet assay; AFLP; RAPD

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Podaci o prilogu

15-15.

2012.

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objavljeno

Podaci o matičnoj publikaciji

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Mommsen, T.P. ; Walsh, P.J.

Bilbao:

1095-6433

Podaci o skupu

28th Congress-European Society for Comparative Physiology and Biochemistry

predavanje

02.09.2012-05.09.2012

Bilbao, Španjolska

Povezanost rada

Biologija

Indeksiranost