Molecular identification of the brown algae, Cystoseira spp. (Phaeophycae, Fucales) from the Adriatic Sea – preliminary results (CROSBI ID 188100)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Rožić, Srđana ; Puizina, Jasna ; Šamanić, Ivica ; Žuljević, Ante ; Antolić, Boris
engleski
Molecular identification of the brown algae, Cystoseira spp. (Phaeophycae, Fucales) from the Adriatic Sea – preliminary results
In the attempt to identify an appropriate molecular marker which will enable genetic distinction between different Cystoseira species from the Adriatic Sea, two mitochondrial molecular markers were tested: the mt 23S rDNA and the mt23S-tRNAVal spacer. Two species were studied: Cystoseira spinosa and Cystoseira squarrosa. Sequence analyses showed no variation in the mt 23S rDNA among all individuals analyzed. But the analysis of the mt23S-tRNAVal spacer showed a differentiation between three haplotypes named A, B and C. The most abundant haplotype A was found in equal number in both species, while haplotype B was found only in C. spinosa and haplotype C was found in C. squarrosa only. However, when comparing to sequences available for several selected Mediterranean Cystoseira species, the mt23S-tRNAVal spacer failed to discriminate between species. Although these results indicate a limited use of the mitochondrial mt23S-tRNAVal intergenic spacer for discrimination among Adriatic Cystoseira species, they could also be interpreted as a sign of conspecificity of the investigated species or the reflection of a recent radiation. Further analysis will be necessary to improve molecular identification of these brown algae.
Cystoseira ; Adriatic Sea ; mitochondrial DNA ; molecular markers ; molecular phylogeny
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