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Apolipoprotein A5 genetic polymorphisms and fasting serum lipidogram in elderly subjects with MetS

Metabolic syndrome (MetS) is a cluster of metabolic abnormalities as are obesity, dyslipidemia, hyperglycemia, and hypertension. It represents a risk factor for many disorders. Apolipoprotein A5 which is located on VLDL and HDL regulates the concentration of triglycerides. Therefore, genetic isoforms might affects triglyceride concentration and contribute in pathogenesis of MetS. The cross-sectional study included 155 men and 187 women older than 70 years. Fasting serum concentration of biochemical parameters were determined by standardized methods. International Diabetes Federation criteria was used for determination of MetS. Two Apo A5 genetics polymorphisms (c.1259T>C-SNP1 andS19W) were genotyped using PCR-RFLP method with detection of fragments with chips on Agilent 2100 bioanalyzer. SNP1 genotype frequencies for T/T, T/C and C/C genotype were 183, 41 and 1 in MetS(+), and 96, 15 and 11 in MetS(-), respectively, (P = 0.478). S19W genotype frequencies for SS, SW and WW were 184, 41 and 2 in MetS(+) and 92, 22 and O in MetS(-), (P = 0.586). Mean total-cholesterol concentration was significantly higher in SNP1-C allele carriers then in non-carriers for women in MetS(+) group (6.23 ± 1.36 and 5.58 ±1.14, respectively, P = 0.020). LDL-cholesterol was also significantly higher for same group and same allele (4.05 ± 1.04 and 3.53 ± 0.97, P = 0.026). In subjects with MetS(+), higher triglycerides concentration were observed in W-carriers than in non-carriers of S19W (median were 1.79 and 1.97, respectively, P = 0.050). Total-cholesterol were higher in men W-carriers vs. non-carriers (5.55 ± 1.17 and 4.97 ± 1.11 respectively, P = 0.048). Apo A5 genetic polymorphisms SNP1 and S19W are associated with dislypidemia in elderly subjects with MetS.

apolipoprotein A5; metabolic syndrome; genetic polymorphism

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