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Pregled bibliografske jedinice broj: 605206

MiR-106a overexpression and pRB downregulation in sporadic colorectal cancer


Catela Ivković, Tina; Aralica, Gorana; Čačev, Tamara; Lončar, Božo; Kapitanović, Sanja
miR-106a overexpression and pRB downregulation in sporadic colorectal cancer // Experimental and molecular pathology, 93 (2013), 1; 148-154 doi:10.1016/j.yexmp.2012.11.002 (međunarodna recenzija, članak, znanstveni)


Naslov
MiR-106a overexpression and pRB downregulation in sporadic colorectal cancer

Autori
Catela Ivković, Tina ; Aralica, Gorana ; Čačev, Tamara ; Lončar, Božo ; Kapitanović, Sanja

Izvornik
Experimental and molecular pathology (0014-4800) 93 (2013), 1; 148-154

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Rb1; miR-106a; expression; pRb

Sažetak
Rb1 plays an important role in cell cycle progression and therefore may be involved in malignant transformation of colonic cells. The aim of our research was to define the potential role of Rb1 as a prognostic biomarker in tumorigenesis of sporadic colorectal cancer, and to examine the role of miR-106a in Rb1 regulation as it functionally binds to 3'UTR of transcribed mRNA. We examined LOH and promoter methylation status. Real-time PCR was used for Rb1 mRNA and miR-106a, and immunohistochemistry for protein expression analysis. All the results obtained from patients' samples were correlated with the clinicopathological parameters in order to determine its influence on the sporadic colorectal carcinogenesis. LOH showed no correlation with mRNA and pRb expression. 51.5% of tumor samples were scored negative for pRb staining. Despite this finding, we detected overexpression of Rb1 mRNA in tumor samples in comparison to the adjacent normal tissue (p=0.023). mRNA overexpression was consistent with Rb1 promoter methylation analysis results, which showed no methylation in the investigated samples. Expression analysis of miR-106a in the patients samples showed its overexpression in colorectal cancer (p<10-4). Negative pRb score was expected according to the definition of tumor suppressor genes and their proposed role in the malignant transformation of the cells. The observed discrepancy between mRNA and protein expression can be explained by a regulatory mechanism that inhibits translation, such as microRNA silencing. Our results suggest that miR-106a might have a regulatory role for Rb1 in sporadic colorectal cancer.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA


Projekt / tema
098-0982464-2508 - Molekularna genetika i farmakogenetika gastrointestinalnih tumora (Sanja Kapitanović, )

Ustanove
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Zagreb,
Klinička bolnica "Dubrava"

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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