engleski

Tissue-specific response of acid DNase activity in mussel Mytilus galloprovincialis exposed to mixed pollutants in sea-water organic extracts

New biomarkers of high sensitivity and low cost are widely investigated for marine biomonitoring purposes. Our previous investigations showed that the exposure of mussels to individual model marine pollutants causes the increase of acid DNase activity in hematocytes and hepatocytes indicating acid DNase activity as a promising biomarker. For further validation mussels were exposed to composite organic extracts of seawater from Adriatic (VRANJIC, industrial area – high potential toxicity, RIJEKA, harbor – moderate potential toxicity, PLOČE, small rural – low potential toxicity) and acid DNase activity was measured in gills and digestive gland. The patterns of influences in both tissues are site specific and related to different potential toxicity of sea-water extracts. In referent mussels acid DNase activity was higher in gills than in digestive gland. The extract from station with low potentional toxicity (PLOČE) did not induce statistically significant change the acid DNase activity in either tissue. Enzyme activity changed in gills of mussels exposed to extract with moderate potential toxicity (RIJEKA) and in digestive gland of mussels exposed to extracts with high potential toxicity (VRANJIC). In both cases enzyme activity increased already after 3 h, remained for 48 h and discriminate stations according to potential toxicity of organic extracts. Advantages/disadvantages of using acid DNase activity in mussel gills and/or digestive gland as biomarker of toxic pollutant presence are discussed.

mussel ; Acid DNase ; marine pollutant ; biomarker

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