engleski

Serotonin transporter polymorphism (5-HTTLPR) in Croatian population

Background: Serotonin transporter is responsible for serotonin reuptake in the brain as well as other tissues . The most studied polymorphism is consisted of 44 base pairs deletion/insertion in the promoter region (5-HTTLPR) that classifies alleles into „short“ (S) or „long“ (L) variant . Short allele is associated with reduced transcriptional activity . Some previous family based studies support thesis of genetic basis of psychiatric disorders . According to previous research, this polymorphism is related to depression and other psychiatric disorders, as well as with response to selective serotonin reuptake inhibitors treatment . Genetic heterogeneity of population might cause misleading association between cases and controls in case- control studies . The aim of this study was to assess frequency of S and L alleles in Croatian population . Materials and methods: 307 healthy individuals of both sexes, (age 18 to 50) were included in this study . Their physical and psychical condition was assessed by physical examination, and MINI psychiatric interview for mental disorders exclusion . Genotyping was performed by simple PCR reaction followed by the gel electrophoresis which is used for separation of 375 bp (allele S) from 419bp (allele L) . Results: Genotype frequencies were in accordance with Hardy-Weinberg equilibrium (P = 0 .114) . L allele frequency is 59% and for S allele 41% . Frequency distribution for studied genotype is: LL – 37% ; LS – 44% ; SS – 19% . Conclusion: Based on Hardy – Weinberg equilibrium, investigated population is homogenous and reported genotype frequencies are in accordance to some other studies performed on European population

Serotonin transporter ; 5-HTTLPR

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