engleski

Quantification and in situ localization of ABCB1 and ABCC9 genes in toxicant-exposed rocky sea urchin (Paracentrotus lividus) embryos

In sea urchin embryos ABC binding cassette (ABC) transport proteins present an efficient defense mechanisms. Still little is known on the expression of these genes during different embryonic stages. Our research focused on real time quantitative PCR (qPCR) measurement and gene localization of two ABC transporters – P-glycoprotein (ABCB1/P-gp) and sulfonylurea receptor like protein (ABCC9/SUR-like), during embryonic development (first 10.5 hours) of P. livdus. Developmental stages (egg, zygote, 2-cell, blastula) were incubated in sub-lethal concentrations of three environmentally relevant pollutants: oxybenzone (50 µM OXI), mercuric chloride (300 nM HgCl2) and trybutiltin (100 pM TBT). In general, we evidenced an increase in mRNA transcript levels of both ABC transporters. Highest increase level was observed in 2-cell stage (ABCB1 - 5.97-fold increase with 50 µM OXI ; ABCC9 - 12.81-fold increase with 300 nM HgCl2) and overall decrease in blastulae stage. In situ hybridization depicted ubiquitous localization of both transcripts within embryo cells, supporting qPCR data and fortifying their protective role in early embryonic development of P. livdus.

sea urchin; ABCB1; ABCC9; xenobiotics

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