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Genetic diversity and population structure of European flat oyster (Ostrea edulis) in the eastern Adriatic Sea

European flat oyster (Ostrea edulis) is one of the commercially most important bivalves in Croatia that is aquacultured and collected from natural populations. Due to problems associated with spread of diseases and overharvesting in certain parts of Mediterranean, wild populations from Adriatic Sea have potentially an important role in establishment of broodstocks for cultured production. Objective of this study was to determine level of haplotype variability and to reconstruct phylogenetic relations between natural and cultured populations of oysters in the eastern Adriatic using molecular methods. Genetic relations between sites were established based on the sequencing of the 12S-rRNA and cytochrome oxidase I(COI) regions. 12S-rRNA region was chosen due to high diversity shown in the previous studies, which appeared to be much more informative than diversity observed for microsatellites, whereas COI region is investigated for the first time in this study. Preliminary results showed high levels of haplotype diversity(h = 0.87) in the mitochondrial 12S-rRNA region, indicating a high level of genetic diversity. A total of 22 polymorphic sites were found, and 19 haplotypes were defined. The results of ANOVA analysis indicated high genetic variation within populations and low genetic variation among populations. The knowledge on genetic diversity and genetic structure will be crucial to establish appropriate broodstock or restoration programmes for this species.

Mediterranean ; marine bivalve ; commercially important ; molecular diversity

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