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Verification of the Body Fluid Application on the Sysmex XE-5000 Hematology Analyzer

Background. Aim of this study is to investigate the performance of BF application on the Sysmex XE-5000 hematology analyzer in differential diagnosis of exudates and transudates. Materials and methods. The XE-5000 BF application provides white blood cell (BF-WBC) and total nucleated cell (BF-TC), as well as erythrocyte (BF-ERC) count. BF-WBC is further subcategorized in mononuclear and polymorphonuclear cells, BF-MN and BF-PMN. Performance of application was evaluated according to CLSI H56-A guidelines. Comparison to reference methods: microscopic cell counting in Neubauer chamber and differential on MGG stained smears after citospin preparation were done. Additionally, measurement of cells on XS2100 analyzer was included in comparative study. Automated cell counting was performed in Institute of Clinical Chemistry and Laboratory Medicine, whilst the microscopic in Institute of Clinical Cytology and Cytogenetics. It was done on various fluids (pleural effusion and ascites, dialysis fluid within peritoneal dialysis treatment) specimens. For correlation calculation Passing and Bablock regression analysis is used. Results. Repeatability of BF-WBC measurements was performed on 3 concentration levels. Coefficients of variation (CVs) were 21, 14 and 7.5, and met manufacturer specifications. BF-WBC linearity was proven for measurement range of 3 to 3420. Achieved coefficients of correlation with microscopic and measured counts on Sysmex XE 2100 for 35 specimens were 0.92 and 0.98, respectively. Obtained coefficient of 0.89 was found in comparison of automated and microscopic differential on 23 samples. Conclusions. Evaluation of BF application on Sysmex XE-5000 showed significant improvement in the ability of automated hematology analyzers regarding body fluid cell count analysis.

body fluid application; verification; hematology analyzer

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