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Reversed-Phase High Performance Liquid Chromatography of Proteins


Josić, Djuro; Kovač, Spomenka
Reversed-Phase High Performance Liquid Chromatography of Proteins // Current Protocols in Protein Science / Dunn, Ben M. (ur.).
online: Wiley Interscience, 2010. str. 8.7.1-8.7.22


Naslov
Reversed-Phase High Performance Liquid Chromatography of Proteins

Autori
Josić, Djuro ; Kovač, Spomenka

Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, znanstveni

Knjiga
Current Protocols in Protein Science

Urednik/ci
Dunn, Ben M.

Izdavač
Wiley Interscience

Grad
Online

Godina
2010

Raspon stranica
8.7.1-8.7.22

ISBN
9780471140863

Ključne riječi
Reversed-phase HPLC, Proteins, Peptides, LC-MS/MS

Sažetak
Reversed-phase HPLC (RP-HPLC) is one of most important techniques for protein separations and method of choice for peptide separation. RP-HPLC has been applied in nano, micro and analytical scale and also scaled up for preparative purifications, up to large industrial scale. Because of its compatibility with mass spectrometry, RP-HPLC is an indispensable tool in proteomic research. With modern instrumentation and columns, complex mixtures of peptides and proteins can be separated at attomolar levels for further analysis. In addition, preparative RP-HPLC is often used for large-scale purification of proteins. This unit provides protocols for packing and testing a column, protein seoaration by use of gradient or step elution, desalting of protein solutions, and separation of enzymatic digests before mass spectrometric analyses. A protocol is also provided for cleaning, regenerating, and storing reversed-phase chromatography columns.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biotehnologija



POVEZANOST RADA


Ustanove
Prehrambeno-tehnološki fakultet, Osijek,
Sveučilište u Osijeku - Odjel za kemiju

Profili:

Avatar Url Spomenka Kovač (autor)

Avatar Url Đuro Josić (autor)

Citiraj ovu publikaciju

Josić, Djuro; Kovač, Spomenka
Reversed-Phase High Performance Liquid Chromatography of Proteins // Current Protocols in Protein Science / Dunn, Ben M. (ur.).
online: Wiley Interscience, 2010. str. 8.7.1-8.7.22
Josić, D. & Kovač, S. (2010) Reversed-Phase High Performance Liquid Chromatography of Proteins. U: Dunn, B. (ur.) Current Protocols in Protein Science. online, Wiley Interscience, str. 8.7.1-8.7.22.
@inbook{inbook, editor = {Dunn, B.}, year = {2010}, pages = {8.7.1-8.7.22}, keywords = {Reversed-phase HPLC, Proteins, Peptides, LC-MS/MS}, isbn = {9780471140863}, title = {Reversed-Phase High Performance Liquid Chromatography of Proteins}, keyword = {Reversed-phase HPLC, Proteins, Peptides, LC-MS/MS}, publisher = {Wiley Interscience}, publisherplace = {online} }