ASSESSMENT OF NITROFURANTOIN AS ECOTOXICANT (CROSBI ID 590747)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
SAUERBORN KLOBUČAR, Roberta ; BROZOVIĆ, Anamaria ; ŠTAMBUK, Anamaria ; ŠRUT, Maja ; TOPIĆ POPOVIĆ, Natalija ; STRUNJAK PEROVIĆ, Ivančica ; Jadan, Margita ; Barišić, Josip ; Čož-Rakovac, Rozelindra
engleski
ASSESSMENT OF NITROFURANTOIN AS ECOTOXICANT
Antibiotic nitrofurantoin (NF) and its derivatives are used in veterinary and human medicine. The impact of NF on the aquatic environment is largely unknown and its ecotoxicity is still under investigation due to confl icting opinions on its use. Increasing evidence suggests that NF can cause various adverse effects in living cells. In this investigation, we used three different fi sh cell lines (PAC2, PLHC-1, R1) to evaluate cytotoxicity of NF towards fi sh. PAC2 cells were shown to be the most sensitive to NF in an MTT colorimetric assay, exhibiting an EC50 of 15 μmol L-1 (3.57 mg L-1) NF. Furthermore, we determined DNA damage and apoptosis in PAC2 cell line depending on the concentration and duration of treatment. We also determined growth inhibition of unicellular algae Scenedesmus subspicatus and mutagenic action towards Salmonella strains (TA98 and TA100) using classic Ames test. A signifi cant decrease in the growth of unicellular algae after 24 h exposure to NF (1 μmol L-1, 10 μmol L-1, 30 μmol L-1, 100 μmol L-1, 200 μmol L-1) was observed. Mutagenicity test responses revealed a large number of revertants following exposure to NF. These results indicate a substantial risk of exposure to NF for the health of aquatic ecosystems
antibiotic; aquatic environment; cytotoxicity; genotoxicity; growth inhibition
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Podaci o prilogu
30-30.
2012.
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objavljeno
Podaci o matičnoj publikaciji
Arhiv za higijenu rada i toksikologiju
Nevenka Kopjar
Zagreb: Denona
0004-1254
Podaci o skupu
4th Croatian Congress of Toxicology (CROTOX 2012)
poster
02.10.2012-05.10.2012
Primošten, Hrvatska
Povezanost rada
Poljoprivreda (agronomija), Biologija