engleski

Acute Myeloid Leukemia with t(6 ; 9)(P23 ; Q34): Case Report

Aim: The translocation (6 ; 9)(p23 ; q34) is a rare cytogenetic aberration found in patients with acute myeloid leukemia (AML) (about 1% of all AML), and correlates with poor clinical outcome. Most patients with this disease are diagnosed with AML according to the FAB classification of M2 or M4, and many show evidence of underlying or preceding myelodysplasia. Methods: Diagnostic bone marrow samples were diagnosed by morphological, immunophenotypical criteria, and reverse transcriptase polymerase chain reaction. Chromosome studies were performed on GTG-banded chromosomes obtained from bone marrow after direct preparation or short-time cultures (24 hour). At least 20 methaphases for each sample were analyzed. FISH studies were done using BCR/ABL1 dual-color/dual_fusion probes (Kreatech, Vysys), 200 interphase nuclei (I-FISH) and on all available methaphases were analysed. Karyotypes and FISH results were described to ISCN (2009). Results: Here we describe a case of AML with t(6 ; 9)(p23 ; q34). A 69-year old male was diagnosed as having AML-M4 based on morphology, cytochemistry and immunophenotype. The blast cells were positive for CD45, CD33, CD13, CD117, CD34, MPO, and negative for CD14, CD4, CD61 and CD235a AML. Cytogenetic analysis of bone marrow showed in all metaphase cells bearing t(6 ; 9) as solitary abnormality in the 20 metaphase cells examened. There was poor response to chemotherapy in that patient, he developed severe sepsis and died in 5 months. Conclusion: It is well recognized that the t(6 ; 9) defines a subgroup of AML with poor outcome. It has been included as a separate entity in the revised 2008 World Health Organization classification of AML.

acute myeloid leukemia; t(6; 9)(p23; q34)

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