engleski

Uptake and impact of engineered nanoparticles on embryonal development and stress response in selected marine organisms

Engineered nanoparticles are finding use in an ever increasing range of consumer and industrial applications ranging from cosmetics and clothing to catalysis and soil remediation. However, questions regarding their accumulation and impact on the environment are, somewhat belatedly, only now being posed. Indeed, data on the impact of such nanoparticles on living organisms in aquatic systems is relatively scarce and the details on the transfer of nanoparticles between trophic levels remain essentially unknown. The aim of this work was therefore to investigate the fate of selected engineered nanoparticles and probe their impact on mussels and sea urchins, both in vivo and in vitro. Here we present preliminary data on the uptake and impact of a range of silver and silica nanoparticles on mussel Mytilus galloprovincialis. Mussels displayed a nanoparticle dose-dependent stress response for nanoparticle concentrations in the 1-100 ppb range on the level of the entire organism (stress-on-stress test ; animal death endpoint), on lysosomal membrane integrity in haemocytes (neutral red leakage from 50% of cells) and on acetylcholinesterase activity in gills. Nanoparticle uptake in gills has been found to be relatively rapid with accumulation of nanoparticles in cells and apparent contact with the nucleus. Further, data on the retardation of embryonal development of Arbacia lixula and Paracentrotus lividus (sea urchin embryo development test) by these nanoparticles at concentrations >50 ppb are also presented.

embryonal development; engineered nanoparticles; M. galloprovincialis; P. lividus; stress response

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