engleski

Influence of hyperbaric oxygen treatment on vascular gene expression in healthy Sprague-Dawley rats

Hyperbaric oxygen treatment (HBOT) involves exposure to 100% oxygen under high pressure (higher than 1 bar). It is well established treatment in many conditions including wound healing, burnings, multiply sclerosis, gangrene, sepsis and others. The mechanism of hyperbaric oxygen therapy is yet unknown. The aim of this pilot study was to determine the influence of HBOT on expression of several genes important in vasomotor function in aorta and cerebral blood vessels of 14 weeks old healthy male Sprague Dawley rats. Rats were not treated (control) or treated with 100% oxygen pressurized to 2 bars, for two hours consecutively for 4 days. On 5th day rats were sacrificed. The relative expression of mRNA was determined by real time PCR. Results showed significant upregulation of CYP4A1 gene in aortas, and CYP4A3 gene in cerebral blood vessels in treated group, compared to controls. Also, the tendency of increased expression in iNOS, eNOS, COX and CYP450-epoxygenase and other CYP450 hydroxylase genes in aortas and cerebral blood vessels were noticed. The tendency of decreased expression in aortal PGI2 and cerebral blood vessel TA2 mRNA was present. These results suggest that HBOT led to upregulation of genes that are important in production of vasodilators and vasoconstrictors.

hyperbaric oxygen treatment; gene expression; vasomotor function



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