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Pregled bibliografske jedinice broj: 596558

Evaluation of three simple direct or indirect carbonyl detection methods for characterization of oxidative modifications of proteins


Vásquez-Garzón, Verónica R.; Rouimi, Patrick; Jouanin, Isabelle; Waeg, Georg; Žarković, Neven; Villa-Treviño, Saul; Guéraud, Françoise
Evaluation of three simple direct or indirect carbonyl detection methods for characterization of oxidative modifications of proteins // Toxicology mechanisms and methods, 22 (2012), 4; 296-304 doi:10.3109/15376516.2012.657258 (međunarodna recenzija, članak, znanstveni)


Naslov
Evaluation of three simple direct or indirect carbonyl detection methods for characterization of oxidative modifications of proteins

Autori
Vásquez-Garzón, Verónica R. ; Rouimi, Patrick ; Jouanin, Isabelle ; Waeg, Georg ; Žarković, Neven ; Villa-Treviño, Saul ; Guéraud, Françoise

Izvornik
Toxicology mechanisms and methods (1537-6516) 22 (2012), 4; 296-304

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Oxidative stress ; carbonylation ; hydroxynonenal

Sažetak
Among disruptions induced by oxidative stress, modifications of proteins, particularly irreversible carbonylation, are associated with the development of several diseases, including cardiovascular diseases, neurodegenerative diseases, and cancer. Carbonylation of proteins can occur directly or indirectly through the adduction of lipid oxidation products. In this study, three classical and easy-to-perform techniques to detect direct or indirect carbonylation of proteins were compared. A model protein apomyoglobin and a complex mixture of rat liver cytosolic proteins were exposed to cumene hydroperoxide oxidation or adduction to the lipid peroxidation product 4-hydroxynonenal in order to test direct or indirect carbonylation, respectively. The technique using a specific anti-4-hydroxynonenal-histidine adduct antibody was effective to detect in vitro modification of model apomyoglobin and cytosolic proteins by 4-hydroxynonenal but not by direct carbonylation which was achieved by techniques using biotin-coupled hydrazide or dinitrophenylhydrazine derivatization of carbonyls. Sequential use of these methods enabled the detection of both direct and indirect carbonyl modification in proteins, although constitutively biotinylated proteins were detected by biotin-hydrazide. Although rather classical and efficient, methods for carbonyl detection on proteins in oxidative stress studies may be biased by some artifactual detections and complicated by proteins multimerizations. The use of more and more specific available antibodies is recommended to complete detection of lipid peroxidation product adducts on proteins.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
098-0982464-2519 - Lipidi, slobodni radikali i njihovi glasnici u integrativnoj onkologiji (Neven Žarković, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Autor s matičnim brojem:
Neven Žarković, (126952)

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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