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Pregled bibliografske jedinice broj: 595571

Removal of aflatoxin M1 from artificially contaminated yoghurt by using of new synthesized dehydroacetic acid analogues


Duraković, Lejla; Delaš, Frane; Tudić, Alma; Huić-Babić, Katarina; Redžepović, Sulejman
Removal of aflatoxin M1 from artificially contaminated yoghurt by using of new synthesized dehydroacetic acid analogues // Mljekarstvo, 62 (2012), 3; 179-191 (međunarodna recenzija, članak, znanstveni)


Naslov
Removal of aflatoxin M1 from artificially contaminated yoghurt by using of new synthesized dehydroacetic acid analogues

Autori
Duraković, Lejla ; Delaš, Frane ; Tudić, Alma ; Huić-Babić, Katarina ; Redžepović, Sulejman

Izvornik
Mljekarstvo (0026-704X) 62 (2012), 3; 179-191

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Aflatoxin M1; yoghurt; dehydroacetic acid analogues; TLC; Artemia salina

Sažetak
Dehydroacetic acid (DHA) and its new synthesized analogues, 4-hydroxy-3-(p-toluoyl)-6-(p-tolyl)-2H-pyrane-2-one (DHT) and 5-Bromo-4-hydroxy-3-(p-toluoyl)-6-(p-tolyl)-2H-pyrane-2-one (BrDHT) were tested for removal of aflatoxin M1 (AFM1) from artificially contaminated yoghurt with known concentrations of this toxin to determine the possible use of these chemicals as a means of controlling AFM1 accumulation. Yoghurt from cow’s milk was artificially contaminated with AFM1 at levels of 0.01 to 0.5 µg/L. Yoghurts were stored at 4 oC and 7 oC, respectively, for up to 28 days. Analysis of AFM1 in yoghurt was carried out using two-dimensional thin-layer chromatography (TLC) – visual estimation. The limit of detection was 0.15 ng/L. The recoveries of AFM1 from the samples spiked at levels of 10, 50, 100, and 500 ng/L were between 80.6 and 107.8%, respectively. Concentrations of DHA and DHT of 0.01 and 0.03 µmol/L had no or little effect on AFM1 content in experimentally contaminated yoghurt, whereas concentrations higher than 0.05 µmol/L, AFM1 content partially inhibited. The percentage loss of the initial AFM1 amount in yoghurt was estimated by about 15 and 25%, and 22 to 45% by the end of storage, respectively. In experiments with 0.01 and 0.05 µmol/L of BrDHT or higher, the concentration of AFM1 was reduced after 28 days by 20 to 95% or completely, respectively, depending on the time and temperature of deposit. Detection of toxicity of investigated analogues was evaluated by using the brine shrimp (Artemia salina) larvae as a screening system for the determination of their sensitivity to some chemicals.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Biotehnologija



POVEZANOST RADA


Projekt / tema
058-0582184-0432 - Mikotoksini u namirnicama i krmi na području R.Hrvatske (Frane Delaš, )
178-1782128-2123 - Mikrobiološka kontrola kvalitete autohonih mekih i tvrdih ovčijih sireva (Sulejman Redžepović, )

Ustanove
Prehrambeno-biotehnološki fakultet, Zagreb,
Agronomski fakultet, Zagreb

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Uključenost u ostale bibliografske baze podataka:


  • AGRICOLA
  • AGRIS International
  • CAB Abstracts
  • CA Search (Chemical Abstracts)
  • FSTA: Food Science and Technology Abstracts
  • EMBiology
  • EBSCO Publishing