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Molecular characterization of high-level mupirocin resistance in Staphylococcus pseudintermedius


Matanović, Krešimir; Perez-Roth, Eduardo; Mekić, Selma; Šeol, Branka
Molecular characterization of high-level mupirocin resistance in Staphylococcus pseudintermedius // CESAR 2012. - book of abstracts / Maravić Vlahoviček Gordana ; Šegvić Klarić Maja ; Abram Maja (ur.).
Zagreb: Croatian Microbiological Society, 2012. str. 38-38 (predavanje, nije recenziran, sažetak, znanstveni)


Naslov
Molecular characterization of high-level mupirocin resistance in Staphylococcus pseudintermedius

Autori
Matanović, Krešimir ; Perez-Roth, Eduardo ; Mekić, Selma ; Šeol, Branka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
CESAR 2012. - book of abstracts / Maravić Vlahoviček Gordana ; Šegvić Klarić Maja ; Abram Maja - Zagreb : Croatian Microbiological Society, 2012, 38-38

ISBN
978-953-7778-04-0

Skup
Central European Symposium on Antimicrobials and Antimicrobial Resistance - CESAR 2012

Mjesto i datum
Primošten, Hrvatska, 23-26.09.2012

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
Molekularna karakterizacija; mupirocin rezistentni sojevi bakterije Staphylococcus pseudintermedius
(Molecular characterization; mupirocin-resistant Staphylococcus pseudintermedius)

Sažetak
Staphylococcus pseudintermedius is one of the most common pathogens isolated from skin and ear infections in dogs. This bacterial species commonly occurs as part of the normal flora of dogs, but can also be an opportunistic pathogen. Mupirocin is a bacteriostatic antibiotic that acts by competitively inhibiting isoleucyl tRNA synthetase. It is frequently used for preoperative elimination of nasal carriage of MRSA in humans and is recommended for treating recurrent interdigital furunculosis, callus pyoderma and muzzle acne in dogs. In S. aureus, resistance to mupirocin is categorized as high-level and low- level. High-level resistance is conferred by a plasmid-associated ileS2 (mupA) gene or a recently described mupB gene, which both encode an alternate isoleucyl-tRNA synthetase. Low-level resistance results from mutations of the native chromosomal isoleucyl-tRNA synthetase (ileS) gene. Resistance to mupirocin in S. pseudintermedius has been reported occasionally, but its molecular basis has not been investigated. We examined 106 S. pseudintermedius isolates for susceptibility to mupirocin using broth microdilution method and found one mupirocin-resistant S. pseudintermedius strain. It showed high-level resistance to mupirocin (MIC >1024 mg/L) and additional resistances to gentamicin, kanamycin, tobramycin and penicillin. We investigated the molecular basis of its mupirocin resistance by a combination of PCR, sequencing and Southern blotting experiments. Southern blotting confirmed that ileS2 and aac6'-aph2'' resistance genes, as well as the conjugative transfer associated traK gene were located on a single pSK41-family plasmid. The analysis of IS257-ileS2 spacer regions revealed a new, UpR849-DnR267 configuration. To our knowledge, this is the first report of high-level mupirocin resistance and of a pSK41-family plasmid carrying ileS2 and aac6' aph2'' gene in S. pseudintermedius.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina



POVEZANOST RADA


Projekt / tema
053-0481153-119

Ustanove
Veterinarski fakultet, Zagreb