hrvatski

Effect of salt stress on Brassica rapa L. seedlings

Povećana koncentracija soli u tlu može nepovoljno utjecati na rast i razvoj biljaka uzrokujući gubitke u poljoprivrednoj proizvodnji. Biljke odgovaraju na solni stres mjenjajući status hormona i uključujući antioksidacijske sustave (antioksidacijski enzimi, glutation i različiti sekundarni metaboliti). Cilj ovog rada bio je istražiti odgovor klijanaca kineskog kupusa (Brassica rapa L. ssp. pekinensis) na stres uzrokovan NaCl (50-200 mM). Inhibicija rasta korijena bila je ovisna o koncentraciji (62 %, 94 %, and 100 % za konc. 50, 100, and 200 mM NaCl, u odnosu na kontrolu). Sadržaj ukupnog glutationa se povećao uslijed NaCl tretmana pri čemu se odnos reducirani GSH/oksidirani GSSG glutation smanjio u odnosu na kontrolu. MDA sadržaj i ukupni fenoli nisu se značajno promjenili uslijed tretmana s 50 i 100 mM NaCl, dok su te vrijednosti povećane u klijancima tretiranim s 200 mM NaCl ukazujući na lipidnu peroksidaciju i toksično djelovanje visoke koncentracije soli. Tretman NaCl-om uzrokovao je slabo povećanje razine H2O2 osim u slučaju tretmana s 200 mM NaCl-om pri čemu smanjenje H2O2 može biti pokazatelj smanjenog metabolizma i ozbiljnih oštećenja klijanaca uslijed stresa. Preliminarne analize biljnih hormona auksina s GC-MS ukazuju na promjene u profile i razini auksina uslijed solnog stresa. Fotosintetski parametri izmjereni OJIP testom pokazali su da fotosintetski aparat odgovara na solni stres smanjnjem fotosintetske efikasnosti. Increased salt concentration in soil may affect plant growth and development causing lost in crop productivity. Plants response to salt stress by changing a hormonal status and by employing an antioxidant systems (antioxidant enzymes, glutathione and various secondary metabolites). The aim of this study was to investigate Chinese cabagge (Brassica rapa L. ssp. pekinensis) seedlings respose to stress induced by NaCl (50 mM to 200 mM). The inhibition of root growth has been observed in a dose dependent manner (62 %, 94 %, and 100 % for conc. of 50, 100, and 200 mM NaCl, respectively, in comparison to control). Level of total glutathione increased upon NaCl treatment with ratio of reduced GSH/oxidized GSSG decreased in comparison to control. MDA content and total phenols identified upon treatments with 50 and 100 mM NaCl were not significantly changed, while those values were increased in seedlings treated with 200 mM NaCl indicated occurrence of lipid peroxidation and toxic effect of the highest NaCl concentration. Salt treatments slightly increased H2O2 levels except 200 mM NaCl treatment in which decreased H2O2 level may be indicator of lower metabolic rate and serious damages of treated seedlings. Preliminary analysis of plant hormone auxin by GC-MS indicated changes in auxin profile and level upon salt stress. Photosynthetic parameters measured by OJIP test showed that photosynthetic apparatus response to salt stress by reducing photosynthetic efficiency.

Brassica rapa L.; solni stress; rast korijena; glutation; fotosinteza

nije evidentirano

engleski

Effect of salt stress on Brassica rapa L. seedlings

Povećana koncentracija soli u tlu može nepovoljno utjecati na rast i razvoj biljaka uzrokujući gubitke u poljoprivrednoj proizvodnji. Biljke odgovaraju na solni stres mjenjajući status hormona i uključujući antioksidacijske sustave (antioksidacijski enzimi, glutation i različiti sekundarni metaboliti). Cilj ovog rada bio je istražiti odgovor klijanaca kineskog kupusa (Brassica rapa L. ssp. pekinensis) na stres uzrokovan NaCl (50-200 mM). Inhibicija rasta korijena bila je ovisna o koncentraciji (62 %, 94 %, and 100 % za konc. 50, 100, and 200 mM NaCl, u odnosu na kontrolu). Sadržaj ukupnog glutationa se povećao uslijed NaCl tretmana pri čemu se odnos reducirani GSH/oksidirani GSSG glutation smanjio u odnosu na kontrolu. MDA sadržaj i ukupni fenoli nisu se značajno promjenili uslijed tretmana s 50 i 100 mM NaCl, dok su te vrijednosti povećane u klijancima tretiranim s 200 mM NaCl ukazujući na lipidnu peroksidaciju i toksično djelovanje visoke koncentracije soli. Tretman NaCl-om uzrokovao je slabo povećanje razine H2O2 osim u slučaju tretmana s 200 mM NaCl-om pri čemu smanjenje H2O2 može biti pokazatelj smanjenog metabolizma i ozbiljnih oštećenja klijanaca uslijed stresa. Preliminarne analize biljnih hormona auksina s GC-MS ukazuju na promjene u profile i razini auksina uslijed solnog stresa. Fotosintetski parametri izmjereni OJIP testom pokazali su da fotosintetski aparat odgovara na solni stres smanjnjem fotosintetske efikasnosti. Increased salt concentration in soil may affect plant growth and development causing lost in crop productivity. Plants response to salt stress by changing a hormonal status and by employing an antioxidant systems (antioxidant enzymes, glutathione and various secondary metabolites). The aim of this study was to investigate Chinese cabagge (Brassica rapa L. ssp. pekinensis) seedlings respose to stress induced by NaCl (50 mM to 200 mM). The inhibition of root growth has been observed in a dose dependent manner (62 %, 94 %, and 100 % for conc. of 50, 100, and 200 mM NaCl, respectively, in comparison to control). Level of total glutathione increased upon NaCl treatment with ratio of reduced GSH/oxidized GSSG decreased in comparison to control. MDA content and total phenols identified upon treatments with 50 and 100 mM NaCl were not significantly changed, while those values were increased in seedlings treated with 200 mM NaCl indicated occurrence of lipid peroxidation and toxic effect of the highest NaCl concentration. Salt treatments slightly increased H2O2 levels except 200 mM NaCl treatment in which decreased H2O2 level may be indicator of lower metabolic rate and serious damages of treated seedlings. Preliminary analysis of plant hormone auxin by GC-MS indicated changes in auxin profile and level upon salt stress. Photosynthetic parameters measured by OJIP test showed that photosynthetic apparatus response to salt stress by reducing photosynthetic efficiency.

Brassica rapa L.; salt stress; root growth; glutathione; photosynthesis

nije evidentirano