engleski

Proteomic approach for comparison of in vitro cultured trypanosomatids Crithidia luciliae and Leishmania infantum

Family Trypanosomatidae consists from several heteroxenous and monoxenous genera, where heteroxenous genera like Trypanosoma, Leishmania and Phytomonas cause serious human, animal and plant diseases, unlike monoxenous genera like Leptomonas, Crithidia, Blastocrithidia and Herpetomonas which inhabit the insects. Members of the genus Leishmania are heteroxenous trypanosomatids, causative agents of cutaneous, mucocutaneous and visceral leishmaniasis in whole world. Leishmania infantum is a causative agent of leishmaniasis in humans and dogs in Mediterranean region and locally in southern parts of Croatia. Source of antigen for serological testing are in vitro grown promastigotes, which are known to be infective for humans during the manipulation and are nutrient demanding. Monoxenous trypanosomatids are in general apathogenic for humans and animals, although they can be pathogenic in immunocompromised human patients. Crithidia genus involves monoxenous parasites which inhabit the digestive tract of insects. Crithidia luciliae is a flagellate in the digestive tract of insects such as common green bottle fly (Lucilia serricata), and house fly (Musca domestica). In general, monoxenous trypanosomatids are less nutrient demanding for in in vitro growth than the pathogenic heteroxenous trypanosomatid species. The cross-reactivity in family Trypanosomatidae is well known phenomenon. A heteroxenous plant trypanosomatid Phytomonas serpens, apathogenic for mammals, share the same antigenic epitopes which are recognized by chagasic sera and are partly protective in Trypanosoma cruzi experimental infection. On the other hand, sera from chagasic patients react with C. luciliae antigen tested with immunofluorescence antibody test and western blotting. Also, sera from Leishmania-infected animals and humans reacted with C. luciliae and L. infantum antigen at the same level when comparatively tested with immunofluorescence antibody test and dot-enzyme linked immunosorbent assay. Promastigotes of all Leishmania species contain on their membrane a glycoprotein known as leishmanolysin or promastigote surface peptidase or major surface peptidase. Homologues of membrane peptidases were described either on the cell surface or being released by apathogenic trypanosomatids belonging to the genera Crithidia, Herpetomonas, Blastocrithidia and Phytomonas. Due to the high level of cross-reactivity and non-specificity in serological tests we compared the proteome profiles of two different species C. luciliae and L. infantum by means of 2-DE coupled with mass spectrometry.

Proteomics; Leishmania infantum; Crithidia luciliae

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