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Structural and Functional Characterization of Ribosomal Protein Gene Introns in Sponges


Perina, Dragutin; Korolija, Marina; Mikoč, Andreja; Roller Milošević, Maša; Pleše, Bruna; Imešek, Mirna; Morrow, Christine; Batel, Renato; Ćetković, Helena
Structural and Functional Characterization of Ribosomal Protein Gene Introns in Sponges // PLoS One, 7 (2012), 8; e42523-e42523 doi:10.1371/journal.pone.0042523 (međunarodna recenzija, članak, znanstveni)


Naslov
Structural and Functional Characterization of Ribosomal Protein Gene Introns in Sponges

Autori
Perina, Dragutin ; Korolija, Marina ; Mikoč, Andreja ; Roller Milošević, Maša ; Pleše, Bruna ; Imešek, Mirna ; Morrow, Christine ; Batel, Renato ; Ćetković, Helena

Izvornik
PLoS One (1932-6203) 7 (2012), 8; E42523-e42523

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Porifera ; snoRNA ; ncRNA ; Demosponges ; small RNA

Sažetak
Ribosomal protein genes (RPGs) are a powerful tool for studying intron evolution. They exist in all three domains of life and are much conserved. Accumulating genomic data suggest that RPG introns in many organisms abound with non-protein-coding-RNAs (ncRNAs). These ancient ncRNAs are small nucleolar RNAs (snoRNAs) essential for ribosome assembly. They are also mobile genetic elements and therefore probably important in diversification and enrichment of transcriptomes through various mechanisms such as intron/exon gain/loss. snoRNAs in basal metazoans are poorly characterized. We examined 449 RPG introns, in total, from four demosponges: Amphimedon queenslandica, Suberites domuncula, Suberites ficus and Suberites pagurorum and showed that RPG introns from A. queenslandica share position conservancy and some structural similarity with “higher” metazoans. Moreover, our study indicates that mobile element insertions play an important role in the evolution of their size. In four sponges 51 snoRNAs were identified. The analysis showed discrepancies between the snoRNA pools of orthologous RPG introns between S. domuncula and A. queenslandica. Furthermore, these two sponges show as much conservancy of RPG intron positions between each other as between themselves and human. Sponges from the Suberites genus show consistency in RPG intron position conservation. However, significant differences in some of the orthologous RPG introns of closely related sponges were observed. This indicates that RPG introns are dynamic even on these shorter evolutionary time scales.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
098-0982913-2874 - Geni i genomi: struktura, funkcija i evolucija (Helena Ćetković, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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