Phenolics in defence response of species of genus Nicotiana infected with Cucumber mosaic virus containing satellite RNA (CROSBI ID 589822)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Likić, Saša ; Rusak, Gordana
engleski
Phenolics in defence response of species of genus Nicotiana infected with Cucumber mosaic virus containing satellite RNA
Nicotiana megalosiphon, N. glutinosa and N. tabacum var. Samsun are used as diagnostic and propagation species in Cucumber mosaic virus (CMV) research. CMV has the widest host range for any plant virus. Some strains of CMV encapsidate evolutionary unrelated satellite RNA (CMVsat). Phenolics are widely distributed among plants and many different physiological roles, including protection of plants against biotic and abiotic stress, have been attributed to them. In order to investigate the differences in response to abiotic and biotic stress within the same species as well as between species of the same genus, changes of phenolics content in species of genus Nicotiana induced by wounding or CMVsat infection were investigated. Analysis of phenolics was performed by high performance liquid chromatography method. Samples were separated on Zorbax Rx-C18 column (75x4, 6 mm, 5 μm, Agilent Technologies) using segmented gradient at 35°C and 1 ml/min flow. Concentration of phenolics in upper untreated leaves was significantly higher compared to lower wounded or virus inoculated leaves. Most of wounded and virus inoculated plants had lower amount of phenolics in all leaves compared to untreated plants. Significant differences of phenolics content in lower or upper leaves established between untreated and wounded as well as between wounded and virus treated plants depended on the time elapsed after the treatment.
plant-pathogen interaction; plant virus; flavonoids; HPLC
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Podaci o prilogu
179-180.
2012.
objavljeno
Podaci o matičnoj publikaciji
Zbornik sažetaka (Hrvatski biološki kongres s međunarodnim sudjelovanjem)
Jelaska, Sven D, Klobučar, Göran IV, Šerić Jelaska, Lucija, Leljak Levanić, Dunja, Lukša, Žaklin
Zagreb: Hrvatsko biološko društvo
1848-5553
Podaci o skupu
11. Hrvatski biološki kongres s međunarodnim sudjelovanjem
poster
16.09.2012-21.09.2012
Šibenik, Hrvatska