Napredna pretraga

Pregled bibliografske jedinice broj: 594288

Turning in to interference : R-loops and Cascade complexes in CRISPR immunity


Ivančić-Baće, Ivana; Howard, Jamieson A.L.; Bolt, Edward L.
Turning in to interference : R-loops and Cascade complexes in CRISPR immunity // Journal of molecular biology, 422 (2012), 5; 607-616 doi:10.1016/j.jmb.2012.06.024 (međunarodna recenzija, pregledni rad, znanstveni)


Naslov
Turning in to interference : R-loops and Cascade complexes in CRISPR immunity

Autori
Ivančić-Baće, Ivana ; Howard, Jamieson A.L. ; Bolt, Edward L.

Izvornik
Journal of molecular biology (0022-2836) 422 (2012), 5; 607-616

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pregledni rad, znanstveni

Ključne riječi
CRISPR; cas3; cascade; R-loop; recombination

Sažetak
Stable RNA-DNA hybrids formed by invasion of an RNA strand into duplex DNA, termed R-loops, are notorious for provoking genome instability especially when they arise during transcription. However, in some instances (DNA replication and class switch recombination), R-loops are useful so long as their existence is carefully managed to avoid them persisting. A recent flow of research papers establishes a newly discovered use for R-loops as key intermediates in a prokaryotic immune system called CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats). Structures and mechanism of ribonucleoprotein complexes (“Cascades”) that form CRISPR R-loops highlight precision targeting of duplex DNA that has sequence characteristics marking it as foe, enabling nucleolytic destruction of DNA and recycling the Cascade. We review these significant recent breakthroughs in understanding targeting/interference stages of CRISPR immunity and discuss questions arising, including a possible link between targeting and adaptive immunity in prokaryotes.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
119-1191196-1201 - Organizacija, funkcija i mehanizmi evolucije biljnog genoma (Višnja Besendorfer, )

Ustanove
Prirodoslovno-matematički fakultet, Zagreb

Autor s matičnim brojem:
Ivana Ivančić Baće, (217264)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati