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Pregled bibliografske jedinice broj: 594219

A novel type of colony formation in marine planktonic diatoms revealed by atomic force microscopy


Bosak, Sunčica; Pletikapić, Galja; Hozić, Amela; Svetličić, Vesna; Sarno, Diana; Viličić, Damir
A novel type of colony formation in marine planktonic diatoms revealed by atomic force microscopy // PLoS One, 7 (2012), 9; e44851, 7 doi:10.1371/journal.pone.0044851 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 594219 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
A novel type of colony formation in marine planktonic diatoms revealed by atomic force microscopy

Autori
Bosak, Sunčica ; Pletikapić, Galja ; Hozić, Amela ; Svetličić, Vesna ; Sarno, Diana ; Viličić, Damir

Izvornik
PLoS One (1932-6203) 7 (2012), 9; E44851, 7

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
diatom ; atomic force microscopy ; bacteriastrum ;

Sažetak
Diatoms have evolved a variety of colonial life forms in which cells are connected by organic threads, mucilage pads or silicate structures. In this study, we provide the first description of a novel strategy of colony formation among marine planktonic diatoms. Bacteriastrum jadranum forms loose but regular chains with distinct heterovalvate terminal cells. The colonial cells and their siliceous projections, the setae, are not in direct contact ; instead, they are enclosed within the optically transparent organic matrix. This cell jacket structure was detected by staining procedure with Alcian Blue, which showed that the polysaccharides are predominant matrix constituents and revealed that the jacket reaches the span of the setae. The scanning electron microscopy (SEM) observations showed distinguishable fibrillar network firmly associated with cells. Using atomic force microscopy (AFM), we were able to visualise and characterise the cell jacket structure at molecular resolution. At nanoscale resolution, the cell jacket appears as a cross-linked fibrillar network organised into a recognisable structure. The circular patches of self-repeating pattern (hexagonal pores with openings of 8–100 nm) are connected through thicker surrounding fibrils and reinforced by branching fibrils. The pore-forming fibrils within the patches are only 0.6–1.6 nm high, the surrounding fibrils connecting patches are 2.0–2.8 nm high, and the branching fibrils are considerably wider but not higher than 4.0 nm. The discovered polysaccharide fibrillar network is highly organised and delicately structured with a monomolecular fibril height of 0.6 nm. We conclude that the Bacteriastrum polysaccharide jacket represents an essential part of the cell, as the conjunction of the polymer network with the frustule appears to be extremely tight and such specific and unique patterns have never been found in self-assembled polysaccharide gel networks, which are usually encountered in the marine environment.

Izvorni jezik
Engleski

Znanstvena područja
Geologija, Biologija



POVEZANOST RADA


Projekti:
098-0982934-2744 - Površinske sile na atomskoj skali u istraživanju mora i nanotehnologiji (Svetličić, Vesna, MZOS ) ( POIROT)
119-1191189-1228 - Struktura i razvoj zajednica mikroalga u trofičkom gradijentu (Viličić, Damir, MZOS ) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Citiraj ovu publikaciju:

Bosak, Sunčica; Pletikapić, Galja; Hozić, Amela; Svetličić, Vesna; Sarno, Diana; Viličić, Damir
A novel type of colony formation in marine planktonic diatoms revealed by atomic force microscopy // PLoS One, 7 (2012), 9; e44851, 7 doi:10.1371/journal.pone.0044851 (međunarodna recenzija, članak, znanstveni)
Bosak, S., Pletikapić, G., Hozić, A., Svetličić, V., Sarno, D. & Viličić, D. (2012) A novel type of colony formation in marine planktonic diatoms revealed by atomic force microscopy. PLoS One, 7 (9), e44851, 7 doi:10.1371/journal.pone.0044851.
@article{article, author = {Bosak, Sun\v{c}ica and Pletikapi\'{c}, Galja and Hozi\'{c}, Amela and Svetli\v{c}i\'{c}, Vesna and Sarno, Diana and Vili\v{c}i\'{c}, Damir}, year = {2012}, pages = {7}, DOI = {10.1371/journal.pone.0044851}, chapter = {e44851}, keywords = {diatom, atomic force microscopy, bacteriastrum, }, journal = {PLoS One}, doi = {10.1371/journal.pone.0044851}, volume = {7}, number = {9}, issn = {1932-6203}, title = {A novel type of colony formation in marine planktonic diatoms revealed by atomic force microscopy}, keyword = {diatom, atomic force microscopy, bacteriastrum, }, chapternumber = {e44851} }
@article{article, author = {Bosak, Sun\v{c}ica and Pletikapi\'{c}, Galja and Hozi\'{c}, Amela and Svetli\v{c}i\'{c}, Vesna and Sarno, Diana and Vili\v{c}i\'{c}, Damir}, year = {2012}, pages = {7}, DOI = {10.1371/journal.pone.0044851}, chapter = {e44851}, keywords = {diatom, atomic force microscopy, bacteriastrum, }, journal = {PLoS One}, doi = {10.1371/journal.pone.0044851}, volume = {7}, number = {9}, issn = {1932-6203}, title = {A novel type of colony formation in marine planktonic diatoms revealed by atomic force microscopy}, keyword = {diatom, atomic force microscopy, bacteriastrum, }, chapternumber = {e44851} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





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