engleski

Identification of Potential Cytogenetical and Biochemical Markers in Bioindication of Common Oak Forests

Cytogenetical and biochemical investigations of common oak (Quercus robur L.) were applied in order to find suitable markers in bioindication of oak populations. Quercus robur karyotype from investigated populations consisted of 24 chromosomes including a satelite pair. One of two small spherical chromatin fragments, B chromosomes were present in some prophase or methaphase cells. Giemsa C-banding, chromomycine A3/DAPI reverse staining and silver impregnation showed the same signal on spherical fragments as on the NORs of satelite chromosomes indicating that B-chromosomes were broken NORs. They were entirely heterochromatic, G-C rich, showing AgNO3 positive reaction and they could be used as markers in bioindication of oak populations. Protein bands of about 60 and 95 kDa were distinct in the leaves of heavily damaged trees. Due to its stability isoperoxidases pattern of dormant buds and young leaves might be convenient tissue for biochemical bioindication.

Quercus robur; bioindication; karyotype; chromosome banding; B-chromosomes; protein pattern; isoperoxidases

nije evidentirano