Napredna pretraga

Pregled bibliografske jedinice broj: 592546

Amyloid-β metabolism in Niemann-Pick C disease models and patients


Mattsson, Niklas; Olsson, Maria; Gustavsson, Mikael K.; Košiček, Marko; Malnar, Martina; Månsson, Jan-Eric; Blomqvist, Maria; Gobom, Johan; Andreasson, Ulf; Brinkmalm, Gunnar et al.
Amyloid-β metabolism in Niemann-Pick C disease models and patients // Metabolic brain disease, 27 (2012), 4; 573-585 doi:10.1007/s11011-012-9332-8 (međunarodna recenzija, članak, znanstveni)


Naslov
Amyloid-β metabolism in Niemann-Pick C disease models and patients

Autori
Mattsson, Niklas ; Olsson, Maria ; Gustavsson, Mikael K. ; Košiček, Marko ; Malnar, Martina ; Månsson, Jan-Eric ; Blomqvist, Maria ; Gobom, Johan ; Andreasson, Ulf ; Brinkmalm, Gunnar ; Vite, Charles ; Hećimović, Silva ; Hastings, Caroline ; Blennow, Kaj ; Zetterberg, Henrik ; Portelius, Erik

Izvornik
Metabolic brain disease (0885-7490) 27 (2012), 4; 573-585

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Niemann-Pick type C; Amyloid-β; Amyloid precursor protein; Biomarker; Cerebrospinal fluid

Sažetak
Niemann-Pick type C (NPC) is a progressive neurodegenerative lysosomal disease with altered cellular lipid trafficking. The metabolism of amyloid-β (Aβ) - previously mainly studied in Alzheimer’s disease - has been suggested to be altered in NPC. Here we aimed to perform a detailed characterization of metabolic products from the amyloid precursor protein (APP) in NPC models and patients. We used multiple analytical technologies, including immunoassays and immunoprecipitation followed by mass spectrometry (IP-MS) to characterize Aβ peptides and soluble APP fragments (sAPP-α/β) in cell media from pharmacologically (U18666A) and genetically (NPC1−/−) induced NPC cell models, and cerebrospinal fluid (CSF) from NPC cats and human patients. The pattern of Aβ peptides and sAPP-α/β fragments in cell media was differently affected by NPC phenotype induced by U18666A treatment and by NPC1−/− genotype. U18666A treatment increased the secreted media levels of sAPP-α, AβX-40 and AβX-42 and reduced the levels of sAPP-β, Aβ1-40 and Aβ1-42, while IP-MS showed increased relative levels ofAβ5-38 and Aβ5-40 in response to treatment. NPC1−/− cells had reduced media levels of sAPP-α and Aβ1-16, and increased levels of sAPP-β. NPC cats had altered CSF distribution of Aβ peptides compared with normal cats. Cats treated with the potential disease-modifying compound 2-hydroxypropyl-β-cyclodextrin had increased relative levels of short Aβ peptides including Aβ1-16 compared with untreated cats. NPC patients receiving β-cyclodextrin had reduced levels over time of CSF Aβ1-42, AβX-38, AβX-40, AβX-42 and sAPP-β, as well as reduced levels of the axonal damage markers tau and phosphorylated tau. We conclude that NPC models have altered Aβ metabolism, but with differences across experimental systems, suggesting that NPC1-loss of function, such as in NPC1−/− cells, or NPC1-dysfunction, seen in NPC patients and cats as well as in U18666A-treated cells, may cause subtle but different effects on APP degradation pathways. The preliminary findings from NPC cats suggest that treatment with cyclodextrin may have an impact on APP processing pathways. CSF Aβ, sAPP and tau biomarkers were dynamically altered over time in human NPC patients.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
098-0982522-2525 - Mehanizam djelovanja kolesterola u nastanku Alzheimerove bolesti (Silva Hećimović, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Citiraj ovu publikaciju

Mattsson, Niklas; Olsson, Maria; Gustavsson, Mikael K.; Košiček, Marko; Malnar, Martina; Månsson, Jan-Eric; Blomqvist, Maria; Gobom, Johan; Andreasson, Ulf; Brinkmalm, Gunnar et al.
Amyloid-β metabolism in Niemann-Pick C disease models and patients // Metabolic brain disease, 27 (2012), 4; 573-585 doi:10.1007/s11011-012-9332-8 (međunarodna recenzija, članak, znanstveni)
Mattsson, N., Olsson, M., Gustavsson, M., Košiček, M., Malnar, M., Månsson, J., Blomqvist, M., Gobom, J., Andreasson, U. & Brinkmalm, G. (2012) Amyloid-β metabolism in Niemann-Pick C disease models and patients. Metabolic brain disease, 27 (4), 573-585 doi:10.1007/s11011-012-9332-8.
@article{article, year = {2012}, pages = {573-585}, DOI = {10.1007/s11011-012-9332-8}, keywords = {Niemann-Pick type C, Amyloid-β, Amyloid precursor protein, Biomarker, Cerebrospinal fluid}, journal = {Metabolic brain disease}, doi = {10.1007/s11011-012-9332-8}, volume = {27}, number = {4}, issn = {0885-7490}, title = {Amyloid-β metabolism in Niemann-Pick C disease models and patients}, keyword = {Niemann-Pick type C, Amyloid-β, Amyloid precursor protein, Biomarker, Cerebrospinal fluid} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati