engleski

Antiphytoviral Activity of Essential Oil of Two Eryngium Species

In this study antiphytoviral activity of essential oils of Eryngium alpinum L. and E. amethystinum L. from Mt Velebit (Croatia) against satellite associated cucumber mosaic virus (satCMV) was investigated. Water distilled essential oils from aerial parts of plants have been analysed by GC and GC/MS using VF-5ms capillary column. Thirty-two components representing 92.4% of the total oil were identified in the essential oil of E. alpinum and thirty-five components representing 93.1% of the total oil of E. amethystinum. The oil of E. alpinum was characterized by a high concentration of caryophyllene oxide (21.6%), bicyclogermacrene (11.8%) and germacrene D (10.3%) while the main components of E. amethystinum oil were β-caryophyllene (19.7%) and α-pinene (12.3%). Germacrene D, caryophyllene oxide and bicyclogermacrene were also identified as the major compounds in the oil of E. serbicum, E. palmatum and E. rosulatum [1, 2]. A comparison (t-test) of the mean number of lesions on the oil-treated Chenopodium quinoa plants with the corresponding control showed that both E. alpinum and E. amethystinum oils significantly reduced infection with satCMV with antiviral activity rate of 77.8% and 80.5%, respectively. Our previous investigation confirmed that sesquiterpenes-rich essential oils as well as oil components β-caryophyllene and caryophyllene oxide inhibit development of local lesions on plants infected with CMV [3-5]. Compared with the oils previously tested and with individuall application of β-caryophyllene and caryophyllene oxide, both Eryngium oils showed stronger inhibition of local lesions development. We can conclude that combination of β-caryophyllene/ caryophyllene oxide with some other oil components resulted with significant antiviral efficiency. Our investigation showed that essential oils isolated from E. alpinum and E. amethystinum posses strong antiviral activity against satCMV infection by inducing resistance response in the host.

β-caryophyllene; caryophyllene oxide; Eryngium alpinum; E. amethystinum; satCMV

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