engleski

Biological effects and phenolic content of felty germander (<i>Teucrium polium</i> L. subsp. <i>polium</i>)

Felty germander - Lamiaceae is popular species of Teucrium genus in the folk medicine and used for treatment of appetite loss and gastrointestinal ailments. In the present study, antioxidative and anti-acetylcholinesterase activity, total phenolic content as well as flavonoid concentration of methanolic, acetone and ethyl acetate extracts were investigated. Ferric reducing/antioxidant power (FRAP) [2] was assayed and values were between 235 and 846µmol Fe 2+ equ/l. The antioxidant capacity have been evaluated using the Briggs-Rauscher oscillating reaction method [3], expressed as a time requierd for regeneration of osscillations in minutes and obtained values were: 43.5 for methanolic, 2.0 for acetone, while ethyl acetate extract did not show activity. The ability to scavenge DPPH radicals [4] was determined and expressed as IC50 values that ranged from 59.37 to 622.96µg/ml. Acetylcholinesterase inhibition was measured using slightly modified Ellman's method [5] and results indicate a weak inhibitory activity of extracts. Total phenolic content was determined using Folin-Ciocalteu reagent and the values ranged from 41.37 up to 124.62mg of GA/g of extract. The content of flavonoids in extracts ranged from 47.76 up to 78.82mg of RU/g of extract. Methanolic extract was most active in comparison with other extracts for all measurments. That indicates that the methanol, as a polar solvent, is the very effective for phenolic compounds extraction from <i>T. polium</i> L. subsp. <i>polium</i>. Based on the obtained results, <i>T. polium</i> subsp. <i>polium</i> extracts are rich sources of phenolic compounds and promising candidates for further development as natural antioxidant agents.

Lamiaceae

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