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Triacylglycerols composition of soybean oil fractions obtained with supercritical CO2

Hexane is widely used as solvent in the processing of vegetable oils ; however, the use of organic solvents cause the environmental problems which can be solved by alternative extraction procedures, such as supercritical extraction with CO2. Soybean oil fractionation with supercritical CO2 at different extraction process conditions was performed. The influence of pressure (300–500 bar), temperature (40–60 °C), CO2 flow rate (0.194–14.125 kg/h) and particle size (0.238–1.059 mm) were investigated. Soybean oil was fractionated into few fractions collected at different time intervals during extraction. The concentration of triacylglycerols in different fractions of soybean oil obtained at different extraction process conditions was determined. Triacylglycerols were analyzed by reversed phase high performance liquid chromatography with refractive infex detector and identified by comparing their retention time to standards. The triacylglycerols composition of soybean oil obtained with supercritical CO2 was compared with the soybean oil extracted by n-hexane. By selecting the relevant process conditions of supercritical extraction, as well as by fractionation, it is possible to obtain soybean oil with different mass concentrations of triacylglycerols. The obtained concentration of main triacylglycerols of soybean oil were as follows: trilinolein (16.34-23.62%), dilinoleoolein (14.61-17.07%), dilinoleopalmitin (10.86-16.82%), and linoleooleopalmitin (11.82-15.44%), depending on extraction conditions.

triacylglycerols; fractions; soybean oil; supercritical CO2 extraction

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