engleski

The Effect of BPC 157 and Gentamycine on Repair Morphodynamic of Alkali Induced Corneal Ulcers in Rabbits

Purpose In this work we have shown the dynamics of histological changes in alkali wounded rabbit cornea during therapy with BPC 157, gentamycine-atropine combination and saline in different groups. Methods Corneas of 30 Chinchilla Bastard rabbits were wounded using 30 application of 2N NaOH through a 9 mm wide plastic tube firmly attached to the eye. Therapy lasted for 17 days and considered of BPC solution in first group, 0, 3% gentamycine and 1% atropine solution in second and saline in the third group, each drug administrated locally. Groups consisted of 10 animals each, and because of monitoring changes during the treatment, extirpation of the eyes was performed on days 3, 6, 9, 14 and 17. In addition, fluoresceine staining was performed every day. Enucleated eyes were fixated in buffered 4% paraformaldehyde during 24 hours, and then cut through the equator and through the lesion margin, and embedded in paraffin. Observations Histologically, initial lesion resulted in necrosis of epithelial and endothelial layer, loss of Bowman capsule, oedema and fiber dissolution of substantia propria, along with vacuolar degeneration and kariorhexis of keratocytes. Descemet membrane was the only undamaged cornea structure. Monitoring the dynamics of histological changes during 17 days showed progressive development of retrocorneal membrane with iridocorneal synechia, together with the presence of vascularized scaring connective tissue on the site of corneal lesion, as two most prominent complications. Neovascularization of various degrees was markedly present in the limbal region. Both complications persisted through the whole treatment period, but development of retrocorneal membrane was significantly lower in the group that received BPC 157 treatment, comparing with other two groups, and also that group did not develop any iridocorneal synechia. Conclusion We have shown that the development of scaring connective tissue along with iridocorneal synechia, being the most important complications after alkali wounded corneas, were significantly reduced after being treated with BPC 157.

BPC-157; gentamycine; corneal ulcers;

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