engleski

Cantharidin-induced inflammation in mouse ear model for translational research of novel anti-inflammatories

The murine model of cantharidin-induced ear inflammation was profiled in detail for its alignment with the human model and to explore the mechanism of antiinflammatory activity of the macrolide antibiotics, clarithromycin and azithromycin. Ear swelling in CD1 mice persisted for 7 days, with peak intensity at 16 h after inflammation induction. As in humans, cantharidin (12.5 mg/ear) generated macrophageinflammatory protein (MIP)-2, monocyte chemoattractant protein (MCP)-1, keratinocyte-derivedchemokine(KC), interleukin (IL)-6, IL-1b, andmyeloperoxidase (MPO) production, as well as neutrophil accumulation in mouse ear tissue. The tested macrolides, clarithromycin and azithromycin, administered orally (2 3 150 mg/kg) 0.5 h before and 5 h after cantharidin challenge, reduced MIP-2, MCP-1, KC, and MPO concentrations and thereby decreased ear swelling. Our results suggest that cantharidin-induced acute inflammation represents an excellent model for translational research of novel anti-inflammatories.

cantharidin; translational; inflamamtion

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