engleski

Growth inhibition and damage of microtubule structure in neuroblastoma cell line due to radiofrequency exposure

Recently published studies recognized that GSM (Global System of Mobile) modulated radiofrequency radiation (RF) may affect biological systems as a result of interference with polar intracellular macromolecular chains (1, 2). The aim of study was to evaluate whether 915 MHz radiofrequency (RF) radiation affects the microtubule cytoskeleton and/or growth of neuroblastoma cell line (SH-SY5Y). RF field was generated inside a Gigahertz Transversal Electromagnetic Mode chamber (GTEM-cell) equipped by generator, power amplifier and signal modulator. Cell samples were exposed to RF field of 915 MHz, electric field strength of 30 V/m. Average specific absorption rate (SAR) was calculated at 1.6 W/kg. Cell exposure treatment lasted for 1, 2, and 3 hours. Irradiated cell groups were matched with negative- and positive control samples. Microtubule proteins were treated immunocytochemicaly, thereafter marked by IgG anti--tubulin (SIGMA Chemical CO, St. Louis, USA) and analyzed using a fluorescent light microscope (1000x magn.). Cell growth was determined by cell counts for each hour of exposure during ten post-exposure days. In comparison with control cells, the microtubule structure clearly altered after 3 hours of irradiation (p<0.05). Significantly decreased growth was noted in cells exposed for two and three hours on third, fourth and fith day after irradiation (p<0.05). GSM modulated radiofrequency radiation (RF) may affect microtubule complex followed by growth impairment of neuroblastoma cell line SH-SY5Y.

growth; SH-SY5Y; microtubule structure; GTEM-cell; radiofrequency (RF) radiation

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