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Pregled bibliografske jedinice broj: 588705

The effects of bluephase LED light on fibroblasts


Ivanišević Malčić, Ana; Pavičić, Ivan; Trošić, Ivančica; Simeon, Paris; Katanec, Davor; Jukić Krmek, Silvana
The effects of bluephase LED light on fibroblasts // European Journal of Dentistry, 6 (2012), 3; 311-317 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 588705 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
The effects of bluephase LED light on fibroblasts

Autori
Ivanišević Malčić, Ana ; Pavičić, Ivan ; Trošić, Ivančica ; Simeon, Paris ; Katanec, Davor ; Jukić Krmek, Silvana

Izvornik
European Journal of Dentistry (1305-7456) 6 (2012), 3; 311-317

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Bluephase effects; LED light; fibroblasts

Sažetak
The aim of this study was to evaluate the effect of Bluephase light emitting diode (LED) light on cell viability, colony-forming ability and proliferation in V79 cell culture and to determine how much the temperature of the nutrient medium rose. The investigation included a low (L), soft start (S) and high (H) illumination mode for 20, 40 and 80 seconds. The viability was determined by the trypan blue exclusion test, colony-forming ability by counting colonies 7 days after exposure and cell proliferation by the cell counts on 5 post-exposure days. The temperature change during illumination was recorded (0.1˚C sensitivity). In each experimental condition, 90-95% of the cells were viable, which was in the same range as the controls. Colony-forming ability was not found to be significantly lower (P<.05). A significant decrease in proliferation was recorded on the 4th post-exposure day with S and H irrespective of time, on the 3 rd day with S for 80 s and H for 40 and 80 s, and with S and H for 80 s on the 2 nd day (P<.05).The temperature rise was significant with S (P<.05) and H (P<.05), irrespective of exposure duration. Dependent on total energy density, LED blue light affects the mitotic activity of cells in its path to a certain extent. Altered mitotic activity was not noted with illumination at the low power mode (intensity of 421.7 ±1.1 mW/cm 2 ). The greatest temperature rise was 8.3 ˚C and occurred at the highest intensity and exposure duration.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Dentalna medicina



POVEZANOST RADA


Projekti:
022-0222411-2406 - BIOLOŠKI POKAZATELJI DJELOVANJA ELEKTROMAGNETSKOG NEIONIZIRAJUĆEG ZRAČENJA (Trošić, Ivančica, MZOS ) ( POIROT)
065-0000000-0424 - Cijeljenje koštanog defekta nakon imedijatne implantacije (Katanec, Davor, MZOS ) ( POIROT)
065-0650445-0434 - Epidemiološki aspekti endodoncije u Republici Hrvatskoj (Krmek, Silvana, MZOS ) ( POIROT)
065-0650446-0435 - Istraživanje keramičkih materijala i alergija u stomatološkoj protetici (Mehulić, Ketij, MZOS ) ( POIROT)

Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Stomatološki fakultet, Zagreb


Citiraj ovu publikaciju

Ivanišević Malčić, Ana; Pavičić, Ivan; Trošić, Ivančica; Simeon, Paris; Katanec, Davor; Jukić Krmek, Silvana
The effects of bluephase LED light on fibroblasts // European Journal of Dentistry, 6 (2012), 3; 311-317 (međunarodna recenzija, članak, znanstveni)
Ivanišević Malčić, A., Pavičić, I., Trošić, I., Simeon, P., Katanec, D. & Jukić Krmek, S. (2012) The effects of bluephase LED light on fibroblasts. European Journal of Dentistry, 6 (3), 311-317.
@article{article, year = {2012}, pages = {311-317}, keywords = {Bluephase effects, LED light, fibroblasts}, journal = {European Journal of Dentistry}, volume = {6}, number = {3}, issn = {1305-7456}, title = {The effects of bluephase LED light on fibroblasts}, keyword = {Bluephase effects, LED light, fibroblasts} }
@article{article, year = {2012}, pages = {311-317}, keywords = {Bluephase effects, LED light, fibroblasts}, journal = {European Journal of Dentistry}, volume = {6}, number = {3}, issn = {1305-7456}, title = {The effects of bluephase LED light on fibroblasts}, keyword = {Bluephase effects, LED light, fibroblasts} }

Časopis indeksira:


  • Scopus





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