The effects of bluephase LED light on fibroblasts (CROSBI ID 185441)
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Podaci o odgovornosti
Ivanišević Malčić, Ana ; Pavičić, Ivan ; Trošić, Ivančica ; Simeon, Paris ; Katanec, Davor ; Jukić Krmek, Silvana
engleski
The effects of bluephase LED light on fibroblasts
The aim of this study was to evaluate the effect of Bluephase light emitting diode (LED) light on cell viability, colony-forming ability and proliferation in V79 cell culture and to determine how much the temperature of the nutrient medium rose. The investigation included a low (L), soft start (S) and high (H) illumination mode for 20, 40 and 80 seconds. The viability was determined by the trypan blue exclusion test, colony-forming ability by counting colonies 7 days after exposure and cell proliferation by the cell counts on 5 post-exposure days. The temperature change during illumination was recorded (0.1˚C sensitivity). In each experimental condition, 90-95% of the cells were viable, which was in the same range as the controls. Colony-forming ability was not found to be significantly lower (P<.05). A significant decrease in proliferation was recorded on the 4th post-exposure day with S and H irrespective of time, on the 3 rd day with S for 80 s and H for 40 and 80 s, and with S and H for 80 s on the 2 nd day (P<.05).The temperature rise was significant with S (P<.05) and H (P<.05), irrespective of exposure duration. Dependent on total energy density, LED blue light affects the mitotic activity of cells in its path to a certain extent. Altered mitotic activity was not noted with illumination at the low power mode (intensity of 421.7 ±1.1 mW/cm 2 ). The greatest temperature rise was 8.3 ˚C and occurred at the highest intensity and exposure duration.
Bluephase effects; LED light; fibroblasts
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