engleski

Proteome profiling of deformed vertebrae in aquarium fish black neon tetra, Hyphesobricon herbertaxelrodi L.

Fish vertebral deformities seize attention since they cause significant financial losses in fish production and processing. Factors causing skeletal disorders are reflection of expressed genotype and phenotype. Deformed fish often suffer from malnutrition and are infection prone, thus presenting potential source of infection for entire hatchery. Proteomic approach enables to set correlation of different factors with vertebral deformities at a molecular level. In this paper proteomic approach has been employed to study differentially expressed proteins in deformed and healthy fish vertebrae. Samples were analyzed by two-dimensional gel electrophoresis (2DE) and proteins were subjected to matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF/TOF). Differentially expressed proteins in healthy vertebrae are reflection of normal physiological processes related to oxidative metabolism and osteogenesis. Except calcium binding proteins, key factors in vertebrae morphogenesis are retinoid x receptors that transduce retinal signal in gene regulation. Identified proteins are promising candidates for further analyses regarding biomarker discovery.

mass spectrometry; bone deformity; fish; proteomics

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