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Pregled bibliografske jedinice broj: 587165

Application of hyphenated analytical techniques for the assessment of the environmental metal exposure


Strižak, Željka; Pröfrock, Daniel; Ivanković, Dušica; Helmholz, Heike; Erk, Marijana
Application of hyphenated analytical techniques for the assessment of the environmental metal exposure // 13th International Chromatography School / Ukić, Š. ; Bolanča, T. (ur.).
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2012. str. 45-45 (poster, nije recenziran, sažetak, znanstveni)


CROSBI ID: 587165 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Application of hyphenated analytical techniques for the assessment of the environmental metal exposure

Autori
Strižak, Željka ; Pröfrock, Daniel ; Ivanković, Dušica ; Helmholz, Heike ; Erk, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
13th International Chromatography School / Ukić, Š. ; Bolanča, T. - Zagreb : University of Zagreb, Faculty of Chemical Engineering and Technology, 2012, 45-45

ISBN
978-953-6470-58-7

Skup
13th International Chromatography School

Mjesto i datum
Zagreb, Hrvatska, 18-19.06.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
metal exposure; SE-HPLC-ICP-MS; cytosolic metal profiles

Sažetak
Depending on their concentration non essential but also essential trace metals and metalloids can cause toxic effects on living organisms. Determination of the total content of selected metals in biological tissue is regularly implemented in environmental monitoring programs, but this is insufficient for the metal exposure assessment. On the other hand, the early effects of metal contamination can be already observed at the molecular and cellular level of an organism chronically exposed to even low doses and in this way may serve as an “early warning” indicator. Response of an organism to metal charge can be achieved by binding metals to various cytosolic biomolecules as ligands. Therefore, the investigation of cytosolic profiles of metals bound to biomolecules of different molecular size can be of great importance as a first step in defining possible biomarkers of metal exposure in the field of pollution monitoring. Coupling size exclusion high performance liquid chromatography (SE-HPLC) with online detection by a sensitive and multi element isotope detector such as an ICP-MS represents an excellent combination which enables obtaining such information. In this contribution cytosolic patterns of metals associated with biomolecules of different molecular size present in the digestive gland of wild mussels (Mytilus galloprovincialis) as a common bioindicator organism were studied. Mussels’ cytosol-protein profiles from several marinas and harbours considered as fairly polluted areas and reference location were compared. Size exclusion chromatography was performed at Superdex 75 (molecular weight range: 3 kDa – 70 kDa) and Superdex 200 (molecular weight range 10 kDa – 600 kDa) in order to obtain profiles for selected metals bound to a wide range of molecular size biomolecules. Metal profiles from more polluted locations have indicated increased intensities in chromatographic fractions related to specific biomolecule size ranges, which are characteristic for selected known metal containing biomarkers for that specific metal (such as metallothionein for Cu, Zn and/or Cd). Preparative chromatography of these fractions can be a starting point for subsequent purification and identification of proteins with the final goal of defining a set of biomarkers specific for metal pollution. This study was performed within the scope of the DAAD project (Project-ID 50752021 Deutscher Akademischer Austausch Dienst) during which scientific collaboration between the research groups from Croatia and Germany has been established.

Izvorni jezik
Engleski

Znanstvena područja
Geologija, Biologija



POVEZANOST RADA


Projekti:
098-0982934-2721 - Stanične promjene u vodnih organizama pobuđene metalima (Erk, Marijana, MZOS ) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Željka Strižak (autor)

Avatar Url Marijana Erk (autor)

Avatar Url Dušica Ivanković (autor)

Citiraj ovu publikaciju:

Strižak, Željka; Pröfrock, Daniel; Ivanković, Dušica; Helmholz, Heike; Erk, Marijana
Application of hyphenated analytical techniques for the assessment of the environmental metal exposure // 13th International Chromatography School / Ukić, Š. ; Bolanča, T. (ur.).
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2012. str. 45-45 (poster, nije recenziran, sažetak, znanstveni)
Strižak, Ž., Pröfrock, D., Ivanković, D., Helmholz, H. & Erk, M. (2012) Application of hyphenated analytical techniques for the assessment of the environmental metal exposure. U: Ukić, Š. & Bolanča, T. (ur.)13th International Chromatography School.
@article{article, author = {Stri\v{z}ak, \v{Z}eljka and Pr\"{o}frock, Daniel and Ivankovi\'{c}, Du\v{s}ica and Helmholz, Heike and Erk, Marijana}, year = {2012}, pages = {45-45}, keywords = {metal exposure, SE-HPLC-ICP-MS, cytosolic metal profiles}, isbn = {978-953-6470-58-7}, title = {Application of hyphenated analytical techniques for the assessment of the environmental metal exposure}, keyword = {metal exposure, SE-HPLC-ICP-MS, cytosolic metal profiles}, publisher = {University of Zagreb, Faculty of Chemical Engineering and Technology}, publisherplace = {Zagreb, Hrvatska} }
@article{article, author = {Stri\v{z}ak, \v{Z}eljka and Pr\"{o}frock, Daniel and Ivankovi\'{c}, Du\v{s}ica and Helmholz, Heike and Erk, Marijana}, year = {2012}, pages = {45-45}, keywords = {metal exposure, SE-HPLC-ICP-MS, cytosolic metal profiles}, isbn = {978-953-6470-58-7}, title = {Application of hyphenated analytical techniques for the assessment of the environmental metal exposure}, keyword = {metal exposure, SE-HPLC-ICP-MS, cytosolic metal profiles}, publisher = {University of Zagreb, Faculty of Chemical Engineering and Technology}, publisherplace = {Zagreb, Hrvatska} }




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