engleski

Cyto- and genotoxicity of mixed indoor Aspergillus versicolor and Stachybotrys chartarum metabolites in human lung adenocarcioma cells A549

To determine a causal relationship between real mixtures of mycotoxins in the indoor environment and particular human health disorders after their inhalation, it is necessary to estimate minimal efective concentration of mycotoxins able to cause (clinical) symptoms in comparison with in vitro models, to choose optimal biological models to study mycotoxin patogenicity and pathophysiology (pulmonary deposition) and to characterize short- and long-term health damages in people under such an influence. In the study presented the metabolic activity of A549 cells was measured by MTT reduction. Cells were treated with exo- and endometabolites extracts of A. versicolor (AV, incl. sterigmatocystin) and S. chartarum (ST ; atranone chemotype)- 5, 10, 20, 40 and 80 ug/mL for 24 h. Combinations of metabolite AV+ST mixture were applied: 20+20, 10+40 and 40+10 ug/mL, 1% DMSO was control vehicle and didnt alter cell viability. All the named toxic variationswere tested for their genotoxicity also by alkaline comet assay and cytokinesis-block micronucleus test. The results were statisticaly analyzed by ANOVA followed by a multiple comparison Tukey test, and the results of micronucleus test were processed by the Pearsons Chi-quadrat test. The level of P<0.05 was considered statistically significant for all calculations. AV+ST endometabolites showed concentration dependent antagonism in the in vitro cyto- and genotoxic effects. The certain synergism in genotoxicity of the exoproducts of proven higher in vivo toxicity- was found.

Sterigamtocystin; atranone chemotype; comet assay; micronuclei

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