engleski

Salivary interleukin-6 and tumor necrosis factor-alpha in patients with chronic and aggressive periodontitis

Aim: The majority of studies examining cytokines as diagnostic biomarkers for periodontal destruction have been conducted using GCF. Saliva is easily available and has the potential as a diagnostic tool for periodontal disease. Previous studies on salivary levels of cytokines were conflicting therefore the aim was to measure salivary concentrations of IL-6 and TNF-α in patients with chronic (GCP) and aggressive (GAgP) periodontitis, compare them between each other and to healthy controls. Material and Methods: Whole saliva samples were collected from 58 subjects: GAgP (n=17), GCP (n=16), and two healthy control groups (n=12, for GAgP ; n=13, for GCP). Periodontal clinical measurements were taken, and IL-6 and TNF-α were measured by ELISA. Results: Significantly lower levels of IL-6 were found in the GAgP group (mean: 27.35 pg/ml) than in the control group (mean: 38.08 pg/ml ; P=0.024). TNF-α showed no statistical significance. Mean salivary levels of TNF-α were significantly lower in the GCP group (mean: 18.81 pg/ml) than in the control group (mean: 30.54 ; P=0.001). IL-6 showed no statistical significance. Spearman rank correlation didn’t reveal correlation between concentrations of IL-6 and TNF-α and clinical parameters (BOP, PD, CAL and teeth number). Conclusion: Our data indicate that salivary levels of IL-6 and TNF-α could not be used as a tool to distinguish GAgP from GCP. The data puts into question the validity of measuring salivary levels of IL-6 and TNF-α as markers of disease activity. Fut

cytokines; periodontitis; saliva

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