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Sample preparation and liquid chromatographic determination of florfenicol in pig plasma


Šandor, Ksenija; Terzić, Svjetlana; Logomerac Šimunec, Vesna
Sample preparation and liquid chromatographic determination of florfenicol in pig plasma // Proceedings of the 22nd IPVS Congress
Jeju, 2012. str. 523-523 (poster, međunarodna recenzija, cjeloviti rad (in extenso), znanstveni)


Naslov
Sample preparation and liquid chromatographic determination of florfenicol in pig plasma

Autori
Šandor, Ksenija ; Terzić, Svjetlana ; Logomerac Šimunec, Vesna

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Proceedings of the 22nd IPVS Congress / - Jeju, 2012, 523-523

Skup
IPVS Congress (22 ; 2012)

Mjesto i datum
Jeju, Koreja, 10.-13.06. 2012.

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Florfenicol; pig plasma; HPLC

Sažetak
Commercial veterinary drugs with florfenicol as active substance were approved worldwide for the treatment of bacterial pneumonia and associated respiratory infections in pigs. The pharmacokinetics of florfenicol in pigs2, 6 and methods for determination of florfenicol in pig tissues4, 5 have been extensively investigated and published. Studies on an availability of florfenicol in the body fluids of animal after administration of reference and generic drugs have not been fully documented. The aim of our study was to determinate florfenicol in pig plasma after intramuscular administration of reference and generic drug by fast and sensitive high performance liquid chromatography method with diode array detection (HPLC-DAD). A protein precipitation was used as technique for samples preparation and its efficacy was evaluated. A simple and robust HPLC-DAD method with protein precipitation as technique for sample preparation was developed for determination of florfenicol in pig plasma. The method showed to be more sensitive then other method3 with good linearity, precision and accuracy and thereby enabling its use in bioequivalence studies. The elimination dynamics of Nuflor® and Floron in pig plasma after intramuscular application were similar. But, the mean plasma concentrations of florfenicol after intramuscular administration of Floron were significantly higher then those after intramuscular administration of Nuflor®. The differences of florfenicol plasma elimination may have been due to different formulation of excipients in the original and generic drug. The characteristics of excipients such as physicochemical properties or solubilization efficacy1 can affect on drug delivery system. Those results are the base for further studies.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina



POVEZANOST RADA


Projekt / tema
048-0481186-1184 - Učinci i sudbina veterinarskih lijekova i vakcina u organizmu životinja (Svjetlana Terzić, )

Ustanove
Hrvatski veterinarski institut, Zagreb