engleski

Expression of TFF3 in epithelia of mouse fetus

AIMS: Trefoil Factor Family 3 protein has an important role in maintenance of epithelial integrity by acting through various mechanisms. In this work we wanted to get insight in TFF3 expression in mouse fetus METHODS: Mouse fetuses, day 15 to 17 were isolated, fixed in 4% paraformaldehyde, paraffin embedded, cut (6µm), and processed for immunohistochemical staining. Primary polyclonal rabbit anti-TFF3 antibody was used, and PBS as a negative control. RESULTUS: TFF3 expression was found in keratinocytes that constitute basal and middle layers of stratified epithelia in developing skin of fetuses and in keratinocytes at the vestibulum of the oral cavity. Goblet cells of the nasal cavity, bronchi and gut also stained positively. Mild signal was found in the epithelium of kidney collecting ducts. Negative controls showed no signal. CONCLUSIONS: TFF3 has been shown to promote migration of epithelial cells in vitro and in vivo, partly by reducing cell to cell adhesion. It participates in immune response, and activates various signaling cascades. TFF3 is also known to affect apoptosis. Since it is expressed in various epithelial tissues during fetal development, it could play an important role in these actively proliferating tissues. Cancers which express or overexpress TFF3 probably only exploit the physiological properties of TFF3 in growth and metastasing, which has diagnostic and potentially therapeutic implications.

epithelial cells; fetal development; mouse; neoplasms; TFF3 protein

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