engleski

Oxidative stress in patients with liver and kidney transplantation

INTRODUCTION: Oxidative stress as a consequence of increased free radicals production and/or exhaustion of antioxidant defense results in a damage of DNA, proteins, lipids and carbohydrates. As such it is considered to be involved in mechanisms of metabolic disorders and disease development. The aim of this study was to analyze oxidative/antioxidative status in patients subjected to organ transplantation. METHODS: 61 patients were included in the study, 50 with liver, and 11 with kidney transplantation. Blood samples were collected on day 0 (before transplantation), and again one month after transplantation. In erythrocytes catalytic concentrations of glutathione peroxidase (eGPx) and superoxide dismutase (eSOD) were measured using commercial kits, and expressed in terms of U/g Hb. Hemoglobin concentrations were determined using commercially available kits. As a marker of the degree of peroxidation, the concentration of substances which react to thiobarbituric acid was measured in serum spectrophotometricaly. Results were calculated and expressed as concentration of malondialdehyde (sMDA). RESULTS: Among 50 liver transplant patients there were 37 males and 13 females in the age from 21 to 69 years. The average body mass index (BMI) was 24.2 kg/m2 (range 15.6 – 36.6 kg/m2). In kidney transplant group there were 5 male and 6 female patients in the age from 28 to 68 years with the average BMI 24.7 kg/m2 (range 18.4 to 35.6 kg/m2). Catalytic concentrations for eGPx were increased on day 0 in patients awaiting kidney transplantation, but after transplantation they went back to normal. Activities for eSOD and sMDA values were within reference values for both groups. For the whole group a significant negative correlation between eGPx and sMDA was found. Furthermore, higher BMI values were observed in ex-smokers. CONCLUSIONS: With the exception of increased eGPx activity in patients with kidney transplant, no increase in parameters of oxidative stress was detected in patients with organ transplantation.

glutathione peroxidase; malondialdehyde; organ transplantation; oxidative stress; superoxide dismutase

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