engleski

Amperometric Uric Acid Biosensor with Improved Analytical Performances Based on Alkaline-Stable H2O2 Transducer

Electrodes modified with Prussian blue containing structurally incorporated Ni2+ ions were employed as the alkaline-stable H2O2 transducers in amperometric uric acid biosensors based on urate oxidase. The employed H2O2 transducers enabled operation of the biosensor at low cathodic potentials and in mild alkaline media, near the optimal pH of urate oxidase. At the pH value of 9.0 and working potential of –0.05 V, the biosensor exhibited a linear response towards uric acid in the concentration range of 2.5 to 200 μM, with the sensitivity of 26.9±0.4 mAM–1cm–2 and detection limit of 0.65 μM. The biosensor was successfully applied for determination of uric acid in samples of commercial dairy products.

uric acid; biosensor; Prussian blue; hydrogen peroxide; alkaline medium

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