engleski

Impact of oxidative stress indicated by thiobarbituric acid reactive substances (TBARS) and protein carbonyl levels (PC) on ethoxyresorufin-O-deethylase (EROD) induction in common carp (Cyprinus carpio)

This study examined the impact of oxidative stress indicated by TBARS and PC, induced by intensive exercise and cadmium chloride (CdCl2) on ethoxyresorufin-O-deethylase (EROD) activity in juvenile carp (Cyprinus carpio). In the first experiment fish were divided into three groups: 1) control, 2) carp exposed to intensive exercise, 3) carp that was not exercised, but previously as well as carp in group 2) received single dose of 3-methylcholantrene (3-MC). Third and sixth day fish were sacrificed and the measurements were conducted. In the second experiment fish divided to 1) control, 2) carp in water containing CdCl2, 3) carp in declorinated tap water (2 and 3 received single dose of 3-MC seventh day after exposure to CdCl2). The carp wer killed 6 days later and livers wer excised for biochemical analyses. In the first experiment, on the sixth day after treatment with 3-MC, results show statistically significant increase in EROD activity in non-exercised carp, while that increase in carp exposed to intensive exercise was significantly lower. Three days after exposure to 3-MC statistically significant increase in TBARS was observed in both exercised and non-exercised carp. Six days after exposure to 3-MC PC levels were significantly higher in exercised carp. Pretreatment with CdCl2, in the second experiment, caused oxidative stress and reduction of EROD activity. Results show linkage between expression of EROD activity and oxidative stress biomarkers and possible influence of oxidative stress on the cell membrane structures, and consequently on EROD activity.

EROD; oxidative stress; lipid peroxidation; protein carbonilation

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