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Pregled bibliografske jedinice broj: 58100

The electric impulses and human interferon-alpha affects growth properties of cellular "microtumors" in vitro


Filipič, Bratko; Toth, S.; Somogyvari, F.; Lacković, Gordana; Valpotić, Ivica; Koren, S.
The electric impulses and human interferon-alpha affects growth properties of cellular "microtumors" in vitro // Congress Book of Abstracts
Tiberias, Izrael, 2000. str. 51-51 (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
The electric impulses and human interferon-alpha affects growth properties of cellular "microtumors" in vitro

Autori
Filipič, Bratko ; Toth, S. ; Somogyvari, F. ; Lacković, Gordana ; Valpotić, Ivica ; Koren, S.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Congress Book of Abstracts / - , 2000, 51-51

Skup
2nd International Conference on Tumor Microenvironment, Progression, Therapy & Prevention

Mjesto i datum
Tiberias, Izrael, 29.10.-01.11.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Sažetak
Exposure of cells to an electric/electromagnetic field produces changes in the synthesis and secretion of different molecules and induces the intracellular metabolic changes. The influence of electric impulses and human interferon-alpha on the growth properties of "microtumors" in vitro of WIREF and HeLa cells was studied. Monolayers in Eagle's medium+10% of FCS were cultivated. To get the "microtumors", cells were seeded into the "bacteriological grade" Petri dishes. After 3-4 days at 37^oC in CO_2 atmosphere, the monolayer cells begin to form the "microtumors" consisting of 30-100 cells. They were sedimented at 500 RPM/10 min. and re-suspended in fresh medium+FCS in a "conc." of cca. 1000 "microtumors"/ml. and exposed to: (1) Direct square-wave impulses of 10 micros duration with 10s break, with a field force of 100 V/cm ; (2) Capacitive impulses at 290 V 1-3 nF of 10micros duration with 10s break ; (3) 10/1000 l.U. /ml of HuIFN-alpha. "Microtumors" were seeded into the tissue-culture grade dishes, and in 3-5 days the atachment and spredding on the surface was followed. AF (alkaline phosphatase) and the NO in the medium were analysed. The data shows: (1) Different effect of inductive and capacitive electric impulses on WiREF and HeLa cells "microtumors" in sense of no. of impulses. (2) 6-9 impulses are needed to inhibit the attachment and growth of microtumors. (3) HuIFN-alpha did not influenced the growth and attachment when added alone. It was effective in combination with 6-9 of inducitve or capative impulses. (4) The level of NO (Nitric oxide) in the medium was reduced after 6-9 impulses only.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina



POVEZANOST RADA


Projekt / tema
053079

Ustanove
Veterinarski fakultet, Zagreb