Overexpression of Tagged Chloroplast Sensor Kinase Confirms its Stromal Localization and Reveals its Possible Involvement in Plant Photomorphogenesis (CROSBI ID 586580)
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Podaci o odgovornosti
Tomašić Paić, Ana ; Marinović, Mila ; Puthiyaveetil, Sujith ; Allen, John F. ; Fulgosi, Hrvoje
engleski
Overexpression of Tagged Chloroplast Sensor Kinase Confirms its Stromal Localization and Reveals its Possible Involvement in Plant Photomorphogenesis
Chloroplast Sensor Kinase (CSK) is a conserved redox sensor kinase, which has been shown to be required for the plastoquinone redox-state dependent regulation of chloroplast reaction centre gene transcription. CSK shows similarity to bacterial family of two-component signalling proteins. The functional partner of CSK in plants and green algae is not a response regulator as in canonical bacterial two-component systems, but an eukaryotic serine/threonine protein kinase known as Plastid Transcription Kinase (PTK) and a chloroplast sigma factor (SIG1). We have generated transgenic Arabidiopsis plants overexpressing CSK tagged with the HA and FLAG peptides. CSK-HA-FLAG plants accumulate tagged CSK in chloroplast stroma which corroborates its possible role in regulation of chloroplast gene expression. In moderate light conditions, CSK-HA-FLAG plants have long leaf petioles and resemble plants grown in low-light or light I conditions. We propose that overexpression of CSK alters redox-dependent regulation of chloroplast origin and reveals novel pathway in plant photomorphogenesis.
Chloroplast Sensor Kinase; two-component signal transduction; photosynthesis; photomorphogenesis
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Podaci o prilogu
2012.
objavljeno
Podaci o matičnoj publikaciji
Podaci o skupu
3rd Congress of Croatian Geneticists with international participation
predavanje
13.05.2012-16.05.2012
Krk, Otok Krk, Hrvatska