Assessment of genetic diversity of wheat genotypes using microsatellite markers (CROSBI ID 184054)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Španić, Valentina ; Buerstmayr, Hermann ; Drezner, Georg
engleski
Assessment of genetic diversity of wheat genotypes using microsatellite markers
Background and Purpose: Genetic diversity is the material basis for crop improvement. In this study, genetic diversity of 30 wheat genotypes was evaluated at the DNA level using 24 simple sequence repeat (SSRs) markers. Materials and Methods: DNA extraction was performed according to the modified CTAB-method. Microsatellite analysis was performed using fluorescent fragment detection on a LI-COR 4200 DNA. Results and Discussion: The number of alleles per locus ranged from 1 to 14 with an average number of 8.44 alleles per locus. The highest number of alleles per locus was detected in the genome A with 7.2, compared to 5.9 and 5.0 for genomes B and D, respectively. The highest number of alleles was recorded at chromosome 7 (9.5), while the lowest number of alleles was detected at chromosomes 3 and 4 (5.0 and 5.3). The smallest genetic distance characterized genotypes Super Zitarka and Zitarka, Tena and Osjecanka, Tena and Bezostaja, Lela and Toras, Janica and Alka, Felix and Seka.Genotypes Pipi and Courtot showed the least genetic similiarities with rest of the genotypes. Conclusions: The identification of genetic diversity should be a good tool of selecting genotypes in breeding programs.
wheat; genetic diversity; microsatellites
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o izdanju
Povezanost rada
Poljoprivreda (agronomija)