engleski

Antimicrobial resistance of coagulase-negative staphylococci isolated from spontaneously fermented wild boar sausages

The aim of this study was to investigate the susceptibility of coagulase negative staphylococci (CoNS) isolated during spontaneous fermentation of wild boar sausages to antimicrobials penicillin, oxacillin, ampicillin, amoxicillin, enrofloxacine, tetracycline, lincomycin, erythromycin, gentamycin, vancomycin and streptomycin. Sausages were sampled at day 0, 14, 21, 35, 43 and 60 of maturation and monitored for total viable count, lactic acid bacteria, enterococci, enterobacteria, Escherichia coli, yeasts, moulds and staphylococci. Each sampling day 15-20 colonies (total of 120 strains) were randomly selected from manitol salt agar. Only 25 isolates were Gram positive, coagulase-negative and catalase positive cocci which were subjected to antimicrobial susceptibility tests by means of disk diffusion and epsilometer (E) method using Mueller Hinton agar and CLSI interpretative breakpoints. All the strains were susceptible to penicillin, oxacillin, ampicillin, amoxicillin, enrofloxacine, gentamycin, vancomycin and streptomycin. In total 16 CoNS strains (64%) showed resistance to tetracycline, erythromycin or lincomycin. Results of this preliminary research indicate a significant prevalence of resistant CoNS strains in spontaneously fermented wild boar sausages.

wild boar sausages; coagulase-negative staphylococci; antimicrobial resistance

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