engleski

Adhesion signals of phospholipid vesicles at an electrified interface

General adhesion behavior of phospholipid vesicles was examined in a wide range of potentials at the mercury electrode by recording time-resolved adhesion signals. It was demonstrated that adhesion-based detection is sensitive to polar head groups in phospholipids vesicles. We have identified a narrow potential window around the point of zero charge of electrode where the interaction of polar head groups of phosphatidylcholine vesicles with the substrate is manifested in the form of bidirectional signals. The bidirectional signal is composed of the charge flow due to the non-specific interaction of vesicle adhesion and spreading, and of the charge flow due to a specific interaction of the negatively charged electrode and the most exposed positively charged choline head groups. These signals are expected to appear only when the electrode surface charge density is less than the surface charge density of the choline groups at the contact interface. In comparison, for the negatively charged phosphatidylserine vesicles, we have identified the potential window at the mercury electrode where charge compensation takes place, and bidirectional signals were not detected.

adhesion signals; choline head group; electrified interface; mercury electrode; phospholipid vesicles

Special Issue on Electroporation – Part 1.