engleski

Potentiometric Study of α-Amylase Kinetics Using a Platinum Redox Sensor

A potentiometric study of α-amylase kinetics using a platinum redox sensor is described in this paper. The sensor measured the amount of triiodide ion released from a starch-triiodide complex after biocatalytic starch degradation, which was correlated with the α-amylase activity. The response characteristics of the platinum redox sensor to the triiodide ion and the working parameters for the starch hydrolysis have been optimized. The kinetics of starch degradation using α-amylase have been measured by direct potentiometry. The two theoretical models for α-amylase kinetics, Michaelis-Menten and Lineweaver-Burk, were calculated from the experimental data and optimized using Solver. The calculated parameter values for α-amylase were Vmax = 2.99E-5 and Km = 0.4862 for the Michaelis-Menten model, and Vmax = 3.31E-05 and Km = 0.5771, Slope = 17449, for the Lineweaver-Burk model.

α-amylase; kinetics; starch-triiodide; direct potentiometry; redox sensor

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